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验证数据展示

  • WB analysis using 66650-1-Ig

    WB analysis using 66650-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66650-1-Ig (RASGRP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 66650-1-Ig

    IF Staining of HEK-293 using 66650-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using RASGRP3 antibody (66650-1-Ig, Clone: 1A10G4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

RASGRP3 Monoclonal antibody

RASGRP3 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 66650-1-Ig

产品说明书

CloneNo. 1A10G4

宿主/亚型

Mouse / IgG2a

种属反应性

Human, mouse

应用

WB, IF/ICC, ELISA

别名

GRP3, KIAA0846, Ras guanyl releasing protein 3, RASGRP3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive WB detected inRaji cells, HEK-293 cells, Jurkat cells, Ramos cells, Raw 264.7 cells
Positive IF/ICC detected inHEK-293 cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66650-1-Ig targets RASGRP3 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse
免疫原 RASGRP3 fusion protein Ag3810 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 RAS guanyl releasing protein 3 (calcium and DAG-regulated)
别名 GRP3, KIAA0846, Ras guanyl releasing protein 3, RASGRP3
计算分子量 690 aa, 78 kDa
观测分子量 78 kDa
GenBank蛋白编号BC027849
基因名称 RASGRP3
Gene ID (NCBI) 25780
RRIDAB_2882007
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8IV61
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AS guanyl releasing protein 3 (RasGRP3) is a member of the RasGRP family and is able to promote guanine nucleotide exchange of Ha-Ras, R-Ras and Rap1 (PMID: 9582122). RasGRP3 is involved in a diverse range of important biological processes. RasGRP3 has emerged as an important mediator of signaling downstream from receptor coupled phosphoinositide turnover in B and T cells. Overexpression of RasGRP3 is commonly associated with many malignancies, such as pre-Bcell leukemia, Burkitt’s lymphoma and natural killer-like T-cell leukemia. Recently, it has been reported that upregulation of RASGRP3 expression in prostate cancer correlates with aggressive capabilities and predicts biochemical recurrence after radical prostatectomy (PMID: 24418912).

实验方案

Product Specific Protocols
WB protocol for RASGRP3 antibody 66650-1-IgDownload protocol
IF protocol for RASGRP3 antibody 66650-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols