• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

RASGRP3 Monoclonal antibody, PBS Only

RASGRP3 Monoclonal Antibody for WB,Indirect ELISA,IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse

Applications

WB,Indirect ELISA,IF

Conjugate

Unconjugated

CloneNo.

1A10G4

Cat No : 66650-1-PBS

Print datasheet

Synonyms

GRP3, KIAA0846, Ras guanyl releasing protein 3, RAS-GRP, RASGRP3

验证数据展示

  • WB analysis using 66650-1-Ig (same clone as 66650-1-PBS)

    WB analysis using 66650-1-Ig (same clone as 66650-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66650-1-Ig (RASGRP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66650-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 66650-1-Ig (same clone as 66650-1-PBS)

    IF Staining of HEK-293 using 66650-1-Ig (same clone as 66650-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using RASGRP3 antibody (66650-1-Ig, Clone: 1A10G4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66650-1-PBS in a different storage buffer formulation.

产品信息

66650-1-PBS targets RASGRP3 in WB, IF, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB,Indirect ELISA,IF Application Description
Tested Reactivity Human, mouse
Immunogen RASGRP3 fusion protein Ag3810 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name RAS guanyl releasing protein 3 (calcium and DAG-regulated)
Synonyms GRP3, KIAA0846, Ras guanyl releasing protein 3, RAS-GRP, RASGRP3
Calculated Molecular Weight 690 aa, 78 kDa
Observed Molecular Weight 78 kDa
GenBank Accession NumberBC027849
Gene Symbol RASGRP3
Gene ID (NCBI) 25780
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8IV61
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

AS guanyl releasing protein 3 (RasGRP3) is a member of the RasGRP family and is able to promote guanine nucleotide exchange of Ha-Ras, R-Ras and Rap1 (PMID: 9582122). RasGRP3 is involved in a diverse range of important biological processes. RasGRP3 has emerged as an important mediator of signaling downstream from receptor coupled phosphoinositide turnover in B and T cells. Overexpression of RasGRP3 is commonly associated with many malignancies, such as pre-Bcell leukemia, Burkitt’s lymphoma and natural killer-like T-cell leukemia. Recently, it has been reported that upregulation of RASGRP3 expression in prostate cancer correlates with aggressive capabilities and predicts biochemical recurrence after radical prostatectomy (PMID: 24418912).