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RASIP1 Polyclonal antibody

RASIP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26064-1-AP

Print datasheet

Synonyms

Ras-interacting protein 1, Ras interacting protein 1, Rain

验证数据展示

  • WB analysis of mouse lung using 26064-1-AP

    WB analysis of mouse lung using 26064-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 26064-1-AP (RASIP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 26064-1-AP

    IHC staining of human heart using 26064-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26064-1-AP (RASIP1 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human heart using 26064-1-AP

    IHC staining of human heart using 26064-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26064-1-AP (RASIP1 Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HepG2 using 26064-1-AP

    IF Staining of HepG2 using 26064-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RASIP1 antibody (26064-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

经过测试的应用

Positive WB detected inmouse lung tissue, NIH/3T3 cells
Positive IHC detected inhuman heart tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26064-1-AP targets RASIP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen RASIP1 fusion protein Ag22036 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Ras interacting protein 1
Synonyms Ras-interacting protein 1, Ras interacting protein 1, Rain
Calculated Molecular Weight 963 aa, 103 kDa
Observed Molecular Weight 115 kDa
GenBank Accession NumberBC021860
Gene Symbol RASIP1
Gene ID (NCBI) 54922
RRIDAB_2880359
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5U651
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RASIP1, also named as Ras-interacting protein 1 or Rain, is a 963 amino acid protein, which contains one dilute domain and one Ras-associating domian. RASIP1 is highly expressed in heart and detected at lower levels in placenta and pancreas. RASIP1 is required for the proper formation of vascular structures that develop via both vasculogenesis and angiogenesis. RASIP1 acts as a critical and vascular-specific regulator of GTPase signaling, cell architecture, and adhesion, which is essential for endothelial cell morphogenesis and blood vessel tubulogenesis.

实验方案

Product Specific Protocols
WB protocol for RASIP1 antibody 26064-1-APDownload protocol
IHC protocol for RASIP1 antibody 26064-1-APDownload protocol
IF protocol for RASIP1 antibody 26064-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols