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验证数据展示

  • WB analysis of mouse lung using 26064-1-AP

    WB analysis of mouse lung using 26064-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 26064-1-AP (RASIP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 26064-1-AP

    IHC staining of human heart using 26064-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26064-1-AP (RASIP1 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human heart using 26064-1-AP

    IHC staining of human heart using 26064-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26064-1-AP (RASIP1 Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HepG2 using 26064-1-AP

    IF Staining of HepG2 using 26064-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RASIP1 antibody (26064-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

RASIP1 Polyclonal antibody

RASIP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 26064-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Ras-interacting protein 1, Ras interacting protein 1, Rain

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse lung tissue, NIH/3T3 cells
Positive IHC detected inhuman heart tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26064-1-AP targets RASIP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RASIP1 fusion protein Ag22036 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Ras interacting protein 1
别名 Ras-interacting protein 1, Ras interacting protein 1, Rain
计算分子量 963 aa, 103 kDa
观测分子量 115 kDa
GenBank蛋白编号BC021860
基因名称 RASIP1
Gene ID (NCBI) 54922
RRIDAB_2880359
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5U651
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RASIP1, also named as Ras-interacting protein 1 or Rain, is a 963 amino acid protein, which contains one dilute domain and one Ras-associating domian. RASIP1 is highly expressed in heart and detected at lower levels in placenta and pancreas. RASIP1 is required for the proper formation of vascular structures that develop via both vasculogenesis and angiogenesis. RASIP1 acts as a critical and vascular-specific regulator of GTPase signaling, cell architecture, and adhesion, which is essential for endothelial cell morphogenesis and blood vessel tubulogenesis.

实验方案

Product Specific Protocols
WB protocol for RASIP1 antibody 26064-1-APDownload protocol
IHC protocol for RASIP1 antibody 26064-1-APDownload protocol
IF protocol for RASIP1 antibody 26064-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols