Phospho-RB1 (Ser780) Recombinant antibody, PBS Only

Phospho-RB1 (Ser780) Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 84692-1-PBS

产品说明书

CloneNo. 241827H7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Indirect ELISA

pRb, RB1, pp110, p110-RB1 , p110-RB1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -


产品信息

84692-1-PBS targets Phospho-RB1 (Ser780) in WB, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 retinoblastoma 1
别名 pRb, RB1, pp110, p110-RB1 , p110-RB1
计算分子量 928 aa, 106 kDa
观测分子量110 kDa
GenBank蛋白编号BC039060
基因名称 RB1
Gene ID (NCBI) 5925
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP06400
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RB1, also named as pp110, pRb and p105 Rb, belongs to the retinoblastoma protein (RB) family. It is a key regulator of entry into cell division that acts as a tumor suppressor. RB1 acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. It is directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. It recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. RB1 controls histone H4 'Lys-20' trimethylation and inhibits the intrinsic kinase activity of TAF1. It mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human RB1.

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