Phospho-RB1 (Ser780) Recombinant antibody

Phospho-RB1 (Ser780) Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 84692-1-RR

产品说明书

CloneNo. 241827H7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

pRb, RB1, pp110, p110-RB1 , p110-RB1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inλ phosphatase treated K-562 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

84692-1-RR targets Phospho-RB1 (Ser780) in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 retinoblastoma 1
别名 pRb, RB1, pp110, p110-RB1 , p110-RB1
计算分子量 928 aa, 106 kDa
观测分子量110 kDa
GenBank蛋白编号BC039060
基因名称 RB1
Gene ID (NCBI) 5925
RRIDAB_3672108
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP06400
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RB1, also named as pp110, pRb and p105 Rb, belongs to the retinoblastoma protein (RB) family. It is a key regulator of entry into cell division that acts as a tumor suppressor. RB1 acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. It is directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. It recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. RB1 controls histone H4 'Lys-20' trimethylation and inhibits the intrinsic kinase activity of TAF1. It mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human RB1.

实验方案

Product Specific Protocols
WB protocol for Phospho-RB1 (Ser780) antibody 84692-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Naunyn Schmiedebergs Arch Pharmacol

Combined Ribociclib and NU7026 administration enhances radio-sensitivity by inhibiting DNA repair in prostate cancer

Authors - Jinbo Sun
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