Phospho-RB1 (Ser780) Recombinant antibody

Phospho-RB1 (Ser780) Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 84692-1-RR

Print datasheet

CloneNo. 241827H7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

pRb, RB1, pp110, p110-RB1 , p110-RB1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -


经过测试的应用

Positive WB detected inλ phosphatase treated K-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

84692-1-RR targets Phospho-RB1 (Ser780) in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name retinoblastoma 1
Synonyms pRb, RB1, pp110, p110-RB1 , p110-RB1
Calculated Molecular Weight 928 aa, 106 kDa
Observed Molecular Weight110 kDa
GenBank Accession NumberBC039060
Gene Symbol RB1
Gene ID (NCBI) 5925
RRIDAB_3672108
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP06400
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RB1, also named as pp110, pRb and p105 Rb, belongs to the retinoblastoma protein (RB) family. It is a key regulator of entry into cell division that acts as a tumor suppressor. RB1 acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. It is directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. It recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. RB1 controls histone H4 'Lys-20' trimethylation and inhibits the intrinsic kinase activity of TAF1. It mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human RB1.

实验方案

Product Specific Protocols
WB protocol for Phospho-RB1 (Ser780) antibody 84692-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Naunyn Schmiedebergs Arch Pharmacol

Combined Ribociclib and NU7026 administration enhances radio-sensitivity by inhibiting DNA repair in prostate cancer

Authors - Jinbo Sun
Loading...