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验证数据展示

  • WB analysis of HEK-293 using 10069-1-AP

    WB analysis of HEK-293 using 10069-1-AP

    WB result of RB1CC1 antibody (10069-1-AP, 1:1000) with si-Control and si-RB1CC1 transfected HEK293 cells.

  • WB analysis of MCF-7 using 10069-1-AP

    WB analysis of MCF-7 using 10069-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 10069-1-AP

    IHC staining of human breast cancer using 10069-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 10069-1-AP (RB1CC1 Antibody) at dilution of 1:50.

    Ready-to-use IHC kits available with RB1CC1 antibody included
  • IHC staining of human appendicitis using 10069-1-AP

    IHC staining of human appendicitis using 10069-1-AP

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 10069-1-AP (RB1CC1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RB1CC1 antibody included
  • IF Staining of HeLa using 10069-1-AP

    IF Staining of HeLa using 10069-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RB1CC1 antibody (10069-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Featured Product
  • KD/KO验证

RB1CC1 Polyclonal antibody

RB1CC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 10069-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

RBICC, RB1-inducible coiled-coil protein 1, RB1CC 1, KIAA0203, FAK family kinase-interacting protein of 200 kDa

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF7 cells, NIH/3T3 cells, HEK-293 cells, HeLa cells, mouse skeletal muscle tissue
Positive IHC detected inhuman appendicitis tissue, human breast cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RB1CC1 Ready-To-Use IHC Kit. RB1CC1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10069-1-AP targets RB1CC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse
文献引用反应性human
免疫原 RB1CC1 fusion protein Ag0003 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RB1-inducible coiled-coil 1
别名 RBICC, RB1-inducible coiled-coil protein 1, RB1CC 1, KIAA0203, FAK family kinase-interacting protein of 200 kDa
计算分子量 1594 aa, 183 kDa
观测分子量 183 kDa
GenBank蛋白编号BC017556
基因名称 RB1CC1
Gene ID (NCBI) 9821
RRIDAB_2877722
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TDY2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RB1CC1, also named as RBICC, is implicated in the regulation of RB1 expression and functions as a DNA-binding transcription factor. It is a potent regulator of the RB1 pathway and a mediator that plays a crucial role in muscular differentiation. Its expression is, thus, a prerequisite for myogenic differentiation. Involved in autophagy. RB1CC1 is required for autophagosome formation. It is probably involved in the tumorigenesis of breast cancer. RB1CC1 is frequently mutated in breast cancer and shows characteristics of a classical tumor suppressor gene. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human RB1CC1.

实验方案

Product Specific Protocols
WB protocol for RB1CC1 antibody 10069-1-APDownload protocol
IHC protocol for RB1CC1 antibody 10069-1-APDownload protocol
IF protocol for RB1CC1 antibody 10069-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Proc Natl Acad Sci U S A

26S proteasomes become stably activated upon heat shock when ubiquitination and protein degradation increase.

Authors - Donghoon Lee
View Article
humanIF

Dev Cell

The RAB11A-Positive Compartment Is a Primary Platform for Autophagosome Assembly Mediated by WIPI2 Recognition of PI3P-RAB11A.

Authors - Claudia Puri
View Article