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验证数据展示

  • FC experiment of HepG2 using CL488-10069

    FC experiment of HepG2 using CL488-10069

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Rb1cc1 Polyclonal Antibody (CL488-10069)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated RB1CC1 Polyclonal antibody

RB1CC1 Polyclonal Antibody for FC (Intra)
Cat No. CL488-10069

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC (Intra)

别名

RBICC, RB1-inducible coiled-coil protein 1, RB1CC 1, KIAA0203, FAK family kinase-interacting protein of 200 kDa

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy RB1CC1 Ready-To-Use IHC Kit. RB1CC1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10069-1-AP

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10069 targets RB1CC1 in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 RB1CC1 fusion protein Ag0003 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RB1-inducible coiled-coil 1
别名 RBICC, RB1-inducible coiled-coil protein 1, RB1CC 1, KIAA0203, FAK family kinase-interacting protein of 200 kDa
计算分子量 1594 aa, 183 kDa
观测分子量 183 kDa
GenBank蛋白编号BC017556
基因名称 RB1CC1
Gene ID (NCBI) 9821
RRIDAB_3672427
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TDY2
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RB1CC1, also named as RBICC, is implicated in the regulation of RB1 expression and functions as a DNA-binding transcription factor. It is a potent regulator of the RB1 pathway and a mediator that plays a crucial role in muscular differentiation. Its expression is, thus, a prerequisite for myogenic differentiation. Involved in autophagy. RB1CC1 is required for autophagosome formation. It is probably involved in the tumorigenesis of breast cancer. RB1CC1 is frequently mutated in breast cancer and shows characteristics of a classical tumor suppressor gene. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human RB1CC1.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 RB1CC1 antibody CL488-10069Download protocol
Standard Protocols
Click here to view our Standard Protocols