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验证数据展示

  • WB analysis using 84757-3-RR

    WB analysis using 84757-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84757-3-RR (RB1CC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 84757-3-RR

    IF Staining of HeLa using 84757-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RB1CC1 antibody (84757-3-RR, Clone: 242204D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84757-3-RR

    Affinity and Kinetic Characterization of 84757-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84757-3-RR against Human RB1CC1 were performed. The affinity constant is 3.20 nM.

RB1CC1 Recombinant antibody

RB1CC1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84757-3-RR

产品说明书

CloneNo. 242204D6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

RB1-inducible coiled-coil protein 1, RB1CC 1, KIAA0203, FAK family kinase-interacting protein of 200 kDa, CC1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, A549 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RB1CC1 Ready-To-Use IHC Kit. RB1CC1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84757-3-RR targets RB1CC1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RB1CC1 fusion protein Ag10508 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RB1-inducible coiled-coil 1
别名 RB1-inducible coiled-coil protein 1, RB1CC 1, KIAA0203, FAK family kinase-interacting protein of 200 kDa, CC1
计算分子量 1594 aa, 183 kDa
观测分子量220 kDa
GenBank蛋白编号BC017556
基因名称 RB1CC1
Gene ID (NCBI) 9821
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ8TDY2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RB1CC1, also named as RBICC, is implicated in the regulation of RB1 expression and functions as a DNA-binding transcription factor. It is a potent regulator of the RB1 pathway and a mediator that plays a crucial role in muscular differentiation. Its expression is, thus, a prerequisite for myogenic differentiation. Involved in autophagy. RB1CC1 is required for autophagosome formation. It is probably involved in the tumorigenesis of breast cancer. RB1CC1 is frequently mutated in breast cancer and shows characteristics of a classical tumor suppressor gene.

实验方案

Product Specific Protocols
WB protocol for RB1CC1 antibody 84757-3-RRDownload protocol
IF protocol for RB1CC1 antibody 84757-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols