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验证数据展示

  • WB analysis using 84330-1-RR (same clone as 84330-1-PBS)

    WB analysis using 84330-1-RR (same clone as 84330-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84330-1-RR (RBBP7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84330-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 84330-1-RR (same clone as 84330-1-PBS)

    IF Staining of A431 using 84330-1-RR (same clone as 84330-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using RBBP7 antibody (84330-1-RR, Clone: 241189D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84330-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 84330-1-RR (same clone as 84330-1-PBS)

    FC experiment of HeLa using 84330-1-RR (same clone as 84330-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RBBP7 Recombinant antibody (84330-1-RR, Clone:241189D6) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100. This data was developed using the same antibody clone with 84330-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 84330-1-RR (same clone as 84330-1-PBS)

    IHC staining of human placenta using 84330-1-RR (same clone as 84330-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84330-1-RR (RBBP7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84330-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RBBP7 antibody included
  • IHC staining of human placenta using 84330-1-RR (same clone as 84330-1-PBS)

    IHC staining of human placenta using 84330-1-RR (same clone as 84330-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84330-1-RR (RBBP7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84330-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RBBP7 antibody included
  • IHC staining of human rectal cancer using 84330-1-RR (same clone as 84330-1-PBS)

    IHC staining of human rectal cancer using 84330-1-RR (same clone as 84330-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 84330-1-RR (RBBP7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84330-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RBBP7 antibody included
  • IHC staining of human rectal cancer using 84330-1-RR (same clone as 84330-1-PBS)

    IHC staining of human rectal cancer using 84330-1-RR (same clone as 84330-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 84330-1-RR (RBBP7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84330-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RBBP7 antibody included

RbAp46 Recombinant monoclonal antibody, PBS Only

RbAp46 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84330-1-PBS

产品说明书

CloneNo. 241189D6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

RBBP7, 241189D6, Histone-binding protein RBBP7, Nucleosome-remodeling factor subunit RBAP46, RBBP-7

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy RBBP7 Ready-To-Use IHC Kit. RBBP7 primary antibody included.

产品信息

84330-1-PBS targets RbAp46 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 retinoblastoma binding protein 7
别名 RBBP7, 241189D6, Histone-binding protein RBBP7, Nucleosome-remodeling factor subunit RBAP46, RBBP-7
计算分子量 48 kDa
观测分子量52 kDa
GenBank蛋白编号NM_002893
基因名称 RBBP7
Gene ID (NCBI) 5931
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ16576
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RBBP7, also named as RBAP46, belongs to the WD repeat RBAP46/RBAP48/MSI1 family. RBBP7 is a core histone-binding subunit that may target chromatin remodeling factors, histone acetyltransferases and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. It is a component of several complexes which regulate chromatin metabolism. The antibody is specific to RBBP7.

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