- Featured Product
- KD/KO Validated
RBBP8 Polyclonal antibody
RBBP8 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | Raji cells, A431 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
产品信息
12624-1-AP targets RBBP8 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Immunogen | RBBP8 fusion protein Ag3314 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | retinoblastoma binding protein 8 |
| Synonyms | CtBP interacting protein, CTIP, DNA endonuclease RBBP8, RBBP 8, RBBP8, RIM, SAE2 |
| Calculated Molecular Weight | 103 kDa |
| Observed Molecular Weight | 120 kDa |
| GenBank Accession Number | BC030590 |
| Gene Symbol | RBBP8 |
| Gene ID (NCBI) | 5932 |
| RRID | AB_2175250 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99708 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RBBP8 is an endonuclease that cooperates with the MRE11-RAD50-NBN (MRN) complex in processing meiotic and mitotic double-strand breaks (DSBs) by ensuring both resection and intrachromosomal association of the broken ends. It functions downstream of the MRN complex and ATM, promotes ATR activation and its recruitment to DSBs in the S/G2 phase facilitating the generation of ssDNA. It interacted with BRCA1 to form a complex that regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage. Also it promotes microhomology-mediated alternative end joining (A-NHEJ) during class-switch recombination and plays an essential role in chromosomal translocations
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RBBP8 antibody 12624-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncogene RBBP8/CtIP suppresses P21 expression by interacting with CtBP and BRCA1 in gastric cancer.
| ||
J Biol Chem PTIP UFMylation promotes replication fork degradation in BRCA1-deficient cells | ||
Cell Biochem Funct The CTIP-mediated repair of TNF-α-induced DNA double-strand break was impaired by miR-130b in cervical cancer cell. | ||
Cell Biosci Modulation of cell cycle increases CRISPR-mediated homology-directed DNA repair |
