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验证数据展示

  • WB analysis using 66015-2-Ig

    WB analysis using 66015-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66015-2-Ig (RBBP9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human pancreas cancer using 66015-2-Ig

    IHC staining of human pancreas cancer using 66015-2-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66015-2-Ig (RBBP9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 66015-2-Ig

    IHC staining of human pancreas cancer using 66015-2-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66015-2-Ig (RBBP9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 66015-2-Ig

    WB analysis using 66015-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66015-2-Ig (RBBP9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 66015-2-Ig

    WB analysis of A549 using 66015-2-Ig

    WB result of RBBP9 antibody (66015-2-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBBP9 transfected A549 cells.

  • FC experiment of HeLa using 66015-2-Ig

    FC experiment of HeLa using 66015-2-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human RBBP9 (66015-2-Ig, Clone:3D5E11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

RBBP9 Monoclonal antibody

RBBP9 Monoclonal Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 66015-2-Ig

产品说明书

CloneNo. 3D5E11

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, pig

应用

WB, IHC, FC (Intra), ELISA

别名

RBBP 10, Glutathione S-Transferase, EC:3.-.-.-, BOG, B5T-overexpressed gene protein

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inpig brain tissue, HeLa cells, A375 cells, A549 cells, rat brain tissue, mouse brain tissue, MCF-7 cells, HepG2 cells, BxPC-3 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66015-2-Ig targets RBBP9 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 RBBP9 fusion protein Ag17864 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 retinoblastoma binding protein 9
别名 RBBP 10, Glutathione S-Transferase, EC:3.-.-.-, BOG, B5T-overexpressed gene protein
计算分子量 186 aa, 21 kDa
观测分子量 22 kDa
GenBank蛋白编号BC015938
基因名称 RBBP9
Gene ID (NCBI) 10741
RRIDAB_11044197
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO75884
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RBBP9, also named as BOG, RBBP10, RBBP-9, RBBP-10 and Protein BOG, belongs to the RBBP9 family. It may play a role in the transformation process due to its capacity to confer resistance to the growth-inhibitory effects of TGF-β1 through interaction with retinoblastoma and the subsequent displacement of E2F-1. RBBP9 is a tumor-associated serine hydrolase activity required for pancreatic neoplasia. It mediates suppression of TGF-β signaling is required for E-cadherin expression as loss of the serine hydrolase activity leads to a reduction in E-cadherin levels and a concomitant decrease in the integrity of tumor cell-cell junctions..RBBP9 protein levels were equivalent in paired primary tumor and nonneoplastic specimens(PMID:20080647)

实验方案

Product Specific Protocols
WB protocol for RBBP9 antibody 66015-2-IgDownload protocol
IHC protocol for RBBP9 antibody 66015-2-IgDownload protocol
FC protocol for RBBP9 antibody 66015-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols