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  • Featured Product
  • KD/KO Validated

RBBP9 Monoclonal antibody, PBS Only

RBBP9 Monoclonal Antibody for Indirect ELISA, IHC, WB

Host / Isotype

Mouse / IgG2b

Reactivity

pig, rat, mouse, human

Applications

Indirect ELISA, IHC, WB

Conjugate

Unconjugated

CloneNo.

3D5E11

Cat no : 66015-2-PBS

Print datasheet

Synonyms

RBBP 10, Glutathione S-Transferase, EC:3.-.-.-, BOG, B5T-overexpressed gene protein

验证数据展示

  • WB analysis using 66015-2-Ig (same clone as 66015-2-PBS)

    WB analysis using 66015-2-Ig (same clone as 66015-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66015-2-Ig (RBBP9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66015-2-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 66015-2-Ig (same clone as 66015-2-PBS)

    IHC staining of human pancreas cancer using 66015-2-Ig (same clone as 66015-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66015-2-Ig (RBBP9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66015-2-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 66015-2-Ig (same clone as 66015-2-PBS)

    IHC staining of human pancreas cancer using 66015-2-Ig (same clone as 66015-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66015-2-Ig (RBBP9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66015-2-PBS in a different storage buffer formulation.

  • WB analysis using 66015-2-Ig (same clone as 66015-2-PBS)

    WB analysis using 66015-2-Ig (same clone as 66015-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66015-2-Ig (RBBP9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66015-2-PBS in a different storage buffer formulation.

  • WB analysis of A549 using 66015-2-Ig (same clone as 66015-2-PBS)

    WB analysis of A549 using 66015-2-Ig (same clone as 66015-2-PBS)

    WB result of RBBP9 antibody (66015-2-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBBP9 transfected A549 cells. This data was developed using the same antibody clone with 66015-2-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 66015-2-Ig (same clone as 66015-2-PBS)

    FC experiment of HeLa using 66015-2-Ig (same clone as 66015-2-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human RBBP9 (66015-2-Ig, Clone:3D5E11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66015-2-PBS in a different storage buffer formulation.

产品信息

66015-2-PBS targets RBBP9 in Indirect ELISA, IHC, WB applications and shows reactivity with pig, rat, mouse, human samples.

Tested Applications Indirect ELISA, IHC, WB Application Description
Tested Reactivity pig, rat, mouse, human
Immunogen RBBP9 fusion protein Ag17864 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name retinoblastoma binding protein 9
Synonyms RBBP 10, Glutathione S-Transferase, EC:3.-.-.-, BOG, B5T-overexpressed gene protein
Calculated Molecular Weight 186 aa, 21 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC015938
Gene Symbol RBBP9
Gene ID (NCBI) 10741
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75884
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RBBP9, also named as BOG, RBBP10, RBBP-9, RBBP-10 and Protein BOG, belongs to the RBBP9 family. It may play a role in the transformation process due to its capacity to confer resistance to the growth-inhibitory effects of TGF-β1 through interaction with retinoblastoma and the subsequent displacement of E2F-1. RBBP9 is a tumor-associated serine hydrolase activity required for pancreatic neoplasia. It mediates suppression of TGF-β signaling is required for E-cadherin expression as loss of the serine hydrolase activity leads to a reduction in E-cadherin levels and a concomitant decrease in the integrity of tumor cell-cell junctions..RBBP9 protein levels were equivalent in paired primary tumor and nonneoplastic specimens(PMID:20080647)