验证数据展示
经过测试的应用
| Positive IF/ICC detected in | A431 cells |
Planning an IHC experiment? We recommend our IHCeasy RBM10 Ready-To-Use IHC Kit. RBM10 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 84104-4-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-84104-4 targets RBM10 in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | RBM10 fusion protein Ag5788 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | RNA binding motif protein 10 |
| 别名 | KIAA0122, GPATCH9, GPATC9, G patch domain-containing protein 9, DXS8237E |
| 计算分子量 | 104 kDa |
| 观测分子量 | 100-120 kDa |
| GenBank蛋白编号 | BC024153 |
| 基因名称 | RBM10 |
| Gene ID (NCBI) | 8241 |
| RRID | AB_3673341 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P98175 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RBM10, also named as RNA-binding protein 10, is a 930 amino acid protein, which is in the extranucleolar nucleoplasm constitutes hundreds of nuclear domains, which dynamically change their structures in a reversible manner. RBM10 may be involved in post-transcriptional processing, most probably in mRNA splicing. RBM10 has some isoforms with MV 100-130 kDa (PMID: 25889998 and PMID: 18315527) .
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 RBM10 antibody CL488-84104-4 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |