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验证数据展示

  • FC experiment of HepG2 using CL488-67506

    FC experiment of HepG2 using CL488-67506

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RBM15B (CL488-67506, Clone:1C2C11) (red), or 0.4 ug x. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated RBM15B Monoclonal antibody

RBM15B Monoclonal Antibody for FC (Intra)
Cat No. CL488-67506

产品说明书

CloneNo. 1C2C11

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

FC (Intra)

别名

RBM15B, RNA-binding motif protein 15B, 1C2C11, HsOTT3, HUMAGCGB, HuOTT3, One-twenty two protein 3, OTT3, Putative RNA-binding protein 15B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy RBM15B Ready-To-Use IHC Kit. RBM15B primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67506-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67506 targets RBM15B in FC (Intra) applications and shows reactivity with Human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human
免疫原 RBM15B fusion protein Ag17853 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 RNA binding motif protein 15B
别名 RBM15B, RNA-binding motif protein 15B, 1C2C11, HsOTT3, HUMAGCGB, HuOTT3, One-twenty two protein 3, OTT3, Putative RNA-binding protein 15B
计算分子量 890 aa, 97 kDa
观测分子量 105 kDa
GenBank蛋白编号BC001367
基因名称 RBM15B
Gene ID (NCBI) 29890
RRIDAB_2923775
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ8NDT2
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RBM15B, one members of the SPEN (Split-end) family of proteins, have repressor function in several signaling pathways and may bind to RNA through interaction with spliceosome components. Present polyclonal anti-RBM15B antibody was produced by immunizing animals with C-terminus of RBM15B, which homolog with a short form of RBM15B(563aa, ~70kDa).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 RBM15B antibody CL488-67506Download protocol
Standard Protocols
Click here to view our Standard Protocols