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RBM17 Recombinant antibody, PBS Only

RBM17 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230125A10

Cat no : 82889-2-PBS

Print datasheet

Synonyms

SPF45, SPF 45, RNA-binding motif protein 17, RBM 17, 45 kDa-splicing factor

验证数据展示

  • IF Staining of HeLa using 82889-2-RR (same clone as 82889-2-PBS)

    IF Staining of HeLa using 82889-2-RR (same clone as 82889-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RBM17 antibody (82889-2-RR, Clone: 230125A10 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82889-2-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 82889-2-RR (same clone as 82889-2-PBS)

    FC experiment of A431 using 82889-2-RR (same clone as 82889-2-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.5 ug Anti-Human RBM17 (82889-2-RR, Clone:230125A10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.5 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 82889-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 82889-2-RR (same clone as 82889-2-PBS)

    IHC staining of mouse testis using 82889-2-RR (same clone as 82889-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82889-2-RR (RBM17 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82889-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 82889-2-RR (same clone as 82889-2-PBS)

    IHC staining of mouse testis using 82889-2-RR (same clone as 82889-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82889-2-RR (RBM17 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82889-2-PBS in a different storage buffer formulation.

  • IP experiment of MCF-7 using 82889-2-RR (same clone as 82889-2-PBS)

    IP experiment of MCF-7 using 82889-2-RR (same clone as 82889-2-PBS)

    IP result of anti-RBM17 (IP:82889-2-RR, 4ug; Detection:82889-2-RR 1:10000) with MCF-7 cells lysate 1470 ug. This data was developed using the same antibody clone with 82889-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82889-2-RR

    Affinity and Kinetic Characterization of 82889-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82889-2-RR against Human RBM17 were performed. The affinity constant is 2.86 nM.

产品信息

82889-2-PBS targets RBM17 in IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
Tested Reactivity human
Immunogen RBM17 fusion protein Ag0985 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name RNA binding motif protein 17
Synonyms SPF45, SPF 45, RNA-binding motif protein 17, RBM 17, 45 kDa-splicing factor
Calculated Molecular Weight 45 kDa
GenBank Accession NumberBC007871
Gene Symbol RBM17
Gene ID (NCBI) 84991
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96I25
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RNA-binding motif protein 17 (RBM17), also named splicing factor 45, was first identified as a member of the spliceosome. RBM17 expression is low in normal tissues but is overexpressed in several forms of cancer, including breast, ovarian and prostate.