验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue |
| Positive IF-P detected in | mouse skeletal muscle tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18178-1-AP targets RBM24 in WB, IHC, IF-P, RIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, RIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, chicken |
| 免疫原 | RBM24 fusion protein Ag12871 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | RNA binding motif protein 24 |
| 别名 | RNA binding motif protein 24, RNA binding protein 24, RNA-binding motif protein 24, RNA-binding protein 24, RNA-binding region-containing protein 6 |
| 计算分子量 | 191 aa, 20 kDa |
| 观测分子量 | 18-25 kDa |
| GenBank蛋白编号 | BC104808 |
| 基因名称 | RBM24 |
| Gene ID (NCBI) | 221662 |
| RRID | AB_2878513 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BX46 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RNA-binding proteins have a pivotal role in post-transcriptionally regulating gene expression. During early development, they exhibit temporally and spatially regulated expression patterns and affect the proteome of a single cell by controlling alternative splicing, transport, localization, and stability of their target RNAs (PMID: 22510371). Their expression is tissue-specific, and their crucial influence on the protein setup, especially in muscle cells, has become recognized recently (PMID: 21982546). RBM24 is one RNA-binding protein that is fundamental in regulating cardiac gene expression, sarcomeric assembly, and cardiac contractility. It may thus represent a novel pathway to cardiomyopathy (PMID: 20338237).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RBM24 antibody 18178-1-AP | Download protocol |
| IF protocol for RBM24 antibody 18178-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Biol Macromol Rbm24 modulates neuronal RNA splicing to restrict cognitive dysfunction | ||
Elife Dual expression of Atoh1 and Ikzf2 promotes transformation of adult cochlear supporting cells into outer hair cells. | ||
Development Mosaic CRISPR-stop enables rapid phenotyping of nonsense mutations in essential genes. | ||
Sci Rep Rbm24 displays dynamic functions required for myogenic differentiation during muscle regeneration.
| ||
Dev Dyn Expression patterns of Rbm24 in lens, nasal epithelium and inner ear during mouse embryonic development. | ||
Mech Dev The RNA-binding protein Rbm24 is transiently expressed in myoblasts and is required for myogenic differentiation during vertebrate development. |