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RBM26 Polyclonal antibody

RBM26 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16823-1-AP

Print datasheet

Synonyms

ARRS2, C13orf10, CTCL tumor antigen se70 2, PRO1777, RBM26, RNA binding motif protein 26, RNA binding protein 26, RP11 255E21.1, SE70 2, ZC3H17

验证数据展示

  • WB analysis using 16823-1-AP

    WB analysis using 16823-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16823-1-AP (RBM26 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 16823-1-AP

    IHC staining of human brain using 16823-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16823-1-AP (RBM26 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human brain using 16823-1-AP

    IHC staining of human brain using 16823-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16823-1-AP (RBM26 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HepG2 using 16823-1-AP

    IF Staining of HepG2 using 16823-1-AP

    Immunofluorescent analysis of HepG2 cells, using RBM26 antibody 16823-1-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • IP experiment of HEK-293 using 16823-1-AP

    IP experiment of HEK-293 using 16823-1-AP

    IP result of anti-RBM26 (IP:16823-1-AP, 4ug; Detection:16823-1-AP 1:5000) with HEK-293 cells lysate 1400 ug.

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16823-1-AP targets RBM26 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen RBM26 fusion protein Ag10398 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RNA binding motif protein 26
Synonyms ARRS2, C13orf10, CTCL tumor antigen se70 2, PRO1777, RBM26, RNA binding motif protein 26, RNA binding protein 26, RP11 255E21.1, SE70 2, ZC3H17
Calculated Molecular Weight 983 aa, 111 kDa
Observed Molecular Weight 125 kDa
GenBank Accession NumberBC000791
Gene Symbol RBM26
Gene ID (NCBI) 64062
RRIDAB_2175942
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5T8P6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RBM26 antibody 16823-1-APDownload protocol
IHC protocol for RBM26 antibody 16823-1-APDownload protocol
IF protocol for RBM26 antibody 16823-1-APDownload protocol
IP protocol for RBM26 antibody 16823-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols