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RBM26 Polyclonal antibody

RBM26 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16823-1-AP

Print datasheet

Synonyms

RNA-binding motif protein 26, RBM 26, PRO1777, CTCL tumor antigen se70-2, C13orf10

验证数据展示

  • WB analysis using 16823-1-AP

    WB analysis using 16823-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16823-1-AP (RBM26 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 16823-1-AP

    IHC staining of human brain using 16823-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16823-1-AP (RBM26 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human brain using 16823-1-AP

    IHC staining of human brain using 16823-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16823-1-AP (RBM26 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HepG2 using 16823-1-AP

    IF Staining of HepG2 using 16823-1-AP

    Immunofluorescent analysis of HepG2 cells, using RBM26 antibody 16823-1-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • IP experiment of HEK-293 using 16823-1-AP

    IP experiment of HEK-293 using 16823-1-AP

    IP result of anti-RBM26 (IP:16823-1-AP, 4ug; Detection:16823-1-AP 1:5000) with HEK-293 cells lysate 1400 ug.

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16823-1-AP targets RBM26 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen RBM26 fusion protein Ag10398 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RNA binding motif protein 26
Synonyms RNA-binding motif protein 26, RBM 26, PRO1777, CTCL tumor antigen se70-2, C13orf10
Calculated Molecular Weight 983 aa, 111 kDa
Observed Molecular Weight 125 kDa
GenBank Accession NumberBC000791
Gene Symbol RBM26
Gene ID (NCBI) 64062
RRIDAB_2175942
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5T8P6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RBM26 antibody 16823-1-APDownload protocol
IHC protocol for RBM26 antibody 16823-1-APDownload protocol
IF protocol for RBM26 antibody 16823-1-APDownload protocol
IP protocol for RBM26 antibody 16823-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

Dual modes of ZFC3H1 confer selectivity in nuclear RNA sorting

Authors - Jing Fan
View Article