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验证数据展示

  • IF Staining of HepG2 using CL594-67420

    IF Staining of HepG2 using CL594-67420

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 RBM39 antibody (CL594-67420, Clone: 2D2C8 ) at dilution of 1:500.

  • FC experiment of HepG2 using CL594-67420

    FC experiment of HepG2 using CL594-67420

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human RBM39 (CL594-67420, Clone:2D2C8) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite®594-conjugated RBM39 Monoclonal antibody

RBM39 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-67420

产品说明书

CloneNo. 2D2C8

宿主/亚型

Mouse / IgG2b

种属反应性

Human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

HCC1, fSAP59, CC1.3, CAPERalpha, CAPER

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy RBM39 Ready-To-Use IHC Kit. RBM39 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67420-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67420 targets RBM39 in IF/ICC, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 Human, mouse, rat
免疫原 RBM39 fusion protein Ag15766 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 RNA binding motif protein 39
别名 HCC1, fSAP59, CC1.3, CAPERalpha, CAPER
计算分子量 530 aa, 59 kDa
观测分子量 60-66 kDa
GenBank蛋白编号BC141835
基因名称 RBM39
Gene ID (NCBI) 9584
RRIDAB_2934766
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ14498
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RBM39, also named as HCC1 or RNPC2, is a 530 amino acid protein, which contains three RRM (RNA recognition motif) domains and belongs to the splicing factor SR family. RBM39 is widely expressed in various tissues and with highly expression in pancreas, skeletal muscle, lung and brain. RBM39 is a transcriptional coactivator for steroid nuclear receptors ESR1/ER-alpha and ESR2/ER-beta, and JUN/AP-1. RBM39 may be involved in pre-mRNA splicing process.

实验方案

Product Specific Protocols
IF protocol for CL594 RBM39 antibody CL594-67420Download protocol
FC protocol for CL594 RBM39 antibody CL594-67420Download protocol
Standard Protocols
Click here to view our Standard Protocols