- Featured Product
- KD/KO Validated
RBM4 Polyclonal antibody
RBM4 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, ELISA and More (4)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, human brain tissue, human heart tissue, human kidney tissue, HeLa cells |
Positive IP detected in | mouse heart tissue |
Positive IHC detected in | human gliomas tissue, human lung cancer tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
11614-1-AP targets RBM4 in WB, IP, IF, FC, RIP, PLA, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IP, IHC, ELISA Application Description |
Cited Applications | WB, IP, IF, FC, RIP, PLA, IHC |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | RBM4 fusion protein Ag2190 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | RNA binding motif protein 4 |
Synonyms | hLark, LARK, Lark homolog, RBM4, RBM4A, RNA binding motif protein 4, RNA binding motif protein 4a, RNA binding protein 4, ZCCHC21, ZCRB3A |
Calculated Molecular Weight | 40 kDa |
Observed Molecular Weight | 40 kDa |
GenBank Accession Number | BC021120 |
Gene Symbol | RBM4 |
Gene ID (NCBI) | 5936 |
RRID | AB_2176095 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9BWF3 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for RBM4 antibody 11614-1-AP | Download protocol |
IHC protocol for RBM4 antibody 11614-1-AP | Download protocol |
IP protocol for RBM4 antibody 11614-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cancer Cell The splicing factor RBM4 controls apoptosis, proliferation, and migration to suppress tumor progression.
| ||
Nucleic Acids Res Modulation of alternative splicing during early infection of human primary B lymphocytes with Epstein-Barr virus (EBV): a novel function for the viral EBNA-LP protein. | ||
Nat Commun A splicing isoform of TEAD4 attenuates the Hippo-YAP signalling to inhibit tumour proliferation. | ||