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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated RBM4 Polyclonal antibody

RBM4 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-11614

Print datasheet

Synonyms

RNA binding protein 4, RNA binding motif protein 4a, RNA binding motif protein 4, RBM4A, Lark homolog

验证数据展示

  • IF Staining of HeLa using CL594-11614

    IF Staining of HeLa using CL594-11614

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594-conjugated RBM4 antibody (CL594-11614) at dilution of 1:100.

  • IF Staining of HepG2 using CL594-11614

    IF Staining of HepG2 using CL594-11614

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594-conjugated RBM4 antibody (CL594-11614) at dilution of 1:100.

  • FC experiment of HeLa using CL594-11614

    FC experiment of HeLa using CL594-11614

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human RBM4 (CL594-11614) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHeLa cells, HepG2 cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 11614-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-11614 targets RBM4 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen RBM4 fusion protein Ag2190 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RNA binding motif protein 4
Synonyms RNA binding protein 4, RNA binding motif protein 4a, RNA binding motif protein 4, RBM4A, Lark homolog
Calculated Molecular Weight 40 kDa
GenBank Accession NumberBC021120
Gene Symbol RBM4
Gene ID (NCBI) 5936
RRIDAB_2919770
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BWF3
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RBM4, RNA-binding motif protein4, contains 2 RRM-type RNA-binding motif and a retroviral-type(RT) zinc finger. RNA-binding factor participates in number of aspects of cellular processes such as alternative splicing of pre-mRNA and translation regulation. RBM4 recruits eIF4A1 to stimulate IRES-dependent translation in responds to cellular stress. Once assembled at the rHRE, the HIF2A-RBM4-eIF4E2 complex captures the 5-prime cap and targets mRNAs to polysomes for active translation, thereby evading hypoxia-induced repression of protein synthesis. This is a rabbit polyclonal antibody raised against the full-length of human RBM4

实验方案

Product Specific Protocols
IF protocol for CL594 RBM4 antibody CL594-11614Download protocol
Standard Protocols
Click here to view our Standard Protocols