• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IF Staining of HeLa using CL594-11614

    IF Staining of HeLa using CL594-11614

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594-conjugated RBM4 antibody (CL594-11614) at dilution of 1:100.

  • IF Staining of HepG2 using CL594-11614

    IF Staining of HepG2 using CL594-11614

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594-conjugated RBM4 antibody (CL594-11614) at dilution of 1:100.

  • FC experiment of HeLa using CL594-11614

    FC experiment of HeLa using CL594-11614

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human RBM4 (CL594-11614) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated RBM4 Polyclonal antibody

RBM4 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-11614

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

RNA binding protein 4, RNA binding motif protein 4a, RNA binding motif protein 4, RBM4A, Lark homolog

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IF/ICC detected inHeLa cells, HepG2 cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 11614-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-11614 targets RBM4 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 RBM4 fusion protein Ag2190 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RNA binding motif protein 4
别名 RNA binding protein 4, RNA binding motif protein 4a, RNA binding motif protein 4, RBM4A, Lark homolog
计算分子量 40 kDa
GenBank蛋白编号BC021120
基因名称 RBM4
Gene ID (NCBI) 5936
RRIDAB_2919770
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BWF3
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RBM4, RNA-binding motif protein4, contains 2 RRM-type RNA-binding motif and a retroviral-type(RT) zinc finger. RNA-binding factor participates in number of aspects of cellular processes such as alternative splicing of pre-mRNA and translation regulation. RBM4 recruits eIF4A1 to stimulate IRES-dependent translation in responds to cellular stress. Once assembled at the rHRE, the HIF2A-RBM4-eIF4E2 complex captures the 5-prime cap and targets mRNAs to polysomes for active translation, thereby evading hypoxia-induced repression of protein synthesis. This is a rabbit polyclonal antibody raised against the full-length of human RBM4

实验方案

Product Specific Protocols
IF protocol for CL594 RBM4 antibody CL594-11614Download protocol
Standard Protocols
Click here to view our Standard Protocols