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RBP4 Recombinant antibody, PBS Only

RBP4 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6O13

Cat no : 82934-1-PBS

Print datasheet

Synonyms

Plasma retinol-binding protein(1-181), Plasma retinol-binding protein(1-179), Plasma retinol-binding protein(1-176), Plasma retinol-binding protein, Plasma retinol binding protein

验证数据展示

  • WB analysis using 82934-1-RR (same clone as 82934-1-PBS)

    WB analysis using 82934-1-RR (same clone as 82934-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82934-1-RR (RBP4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82934-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 82934-1-RR (same clone as 82934-1-PBS)

    FC experiment of HepG2 using 82934-1-RR (same clone as 82934-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug RBP4 Recombinant antibody (82934-1-RR, Clone:6O13) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82934-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82934-1-RR

    Affinity and Kinetic Characterization of 82934-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82934-1-RR against Human RBP4 were performed. The affinity constant is below 1 pM.

Planning an IHC experiment? We recommend our IHCeasy RBP4 Ready-To-Use IHC Kit. RBP4 primary antibody included.

产品信息

82934-1-PBS targets RBP4 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen RBP4 fusion protein Ag2448 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name retinol binding protein 4, plasma
Synonyms Plasma retinol-binding protein(1-181), Plasma retinol-binding protein(1-179), Plasma retinol-binding protein(1-176), Plasma retinol-binding protein, Plasma retinol binding protein
Calculated Molecular Weight 201 aa, 23 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC020633
Gene Symbol RBP4
Gene ID (NCBI) 5950
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP02753
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RBP4 (retinol-binding protein 4) is a carrier protein that transports vitamin A (retinol) from the liver to the peripheral tissues. Synthesized primarily by hepatocytes and adipocytes as a 21 kDa non-glycosylated protein, RBP4 is secreted into the circulation as a retinol-RBP4 complex. In plasma the RBP4-retinol complex is bound to transthyretin (TRR), which prevents prevent kidney filtration. Two truncated forms of RBP4, RBP4-L (truncated at Leu-183) and RBP4-LL (truncated at Leu-182 and Leu-183), exist by proteolytic process. RBP4-L and RBP4-LL, which do not bind TTR, are normally excreted into the urine but accumulate in the serum during renal failure. Urinary RBP4 has been reported as marker for glomerular disease. RBP4 also was identified as an adipokine that elevated in some INS-resistant states. Measurement of serum RBP4 could be used to assess the risk of INS resistance, type 2 diabetes, obesity, and cardiovascular disease. (18752671, 16034410)