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验证数据展示

  • IF Staining of human liver cancer using CL594-66104

    IF Staining of human liver cancer using CL594-66104

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594 RBP4 antibody (CL594-66104, Clone: 1D12B11 ) at dilution of 1:200.

  • IF Staining of HepG2 using CL594-66104

    IF Staining of HepG2 using CL594-66104

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 RBP4 antibody (CL594-66104, Clone: 1D12B11 ) at dilution of 1:100.

CoraLite®594-conjugated RBP4 Monoclonal antibody

RBP4 Monoclonal Antibody for IF/ICC, IF-P
Cat No. CL594-66104

Print datasheet

CloneNo. 1D12B11

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC, IF-P

Synonyms

Plasma retinol-binding protein(1-181), Plasma retinol-binding protein(1-179), Plasma retinol-binding protein(1-176), Plasma retinol-binding protein, Plasma retinol binding protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman liver cancer tissue
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy RBP4 Ready-To-Use IHC Kit. RBP4 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66104-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66104 targets RBP4 in IF/ICC, IF-P applications and shows reactivity with human samples.

Tested Applications IF/ICC, IF-P Application Description
Tested Reactivity human
Immunogen RBP4 fusion protein Ag19295 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name retinol binding protein 4, plasma
Synonyms Plasma retinol-binding protein(1-181), Plasma retinol-binding protein(1-179), Plasma retinol-binding protein(1-176), Plasma retinol-binding protein, Plasma retinol binding protein
Calculated Molecular Weight 201 aa, 23 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC020633
Gene Symbol RBP4
Gene ID (NCBI) 5950
RRIDAB_2919947
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP02753
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RBP4 (retinol-binding protein 4) is a carrier protein that transports vitamin A (retinol) from the liver to the peripheral tissues. Synthesized primarily by hepatocytes and adipocytes as a 21 kDa non-glycosylated protein, RBP4 is secreted into the circulation as a retinol-RBP4 complex. In plasma the RBP4-retinol complex is bound to transthyretin (TRR), which prevents prevent kidney filtration. Two truncated forms of RBP4, RBP4-L (truncated at Leu-183) and RBP4-LL (truncated at Leu-182 and Leu-183), exist by proteolytic process. RBP4-L and RBP4-LL, which do not bind TTR, are normally excreted into the urine but accumulate in the serum during renal failure. Urinary RBP4 has been reported as marker for glomerular disease. RBP4 also was identified as an adipokine that elevated in some INS-resistant states. Measurement of serum RBP4 could be used to assess the risk of INS resistance, type 2 diabetes, obesity, and cardiovascular disease. (18752671, 16034410)

实验方案

Product Specific Protocols
IF protocol for CL594 RBP4 antibody CL594-66104Download protocol
Standard Protocols
Click here to view our Standard Protocols