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验证数据展示

  • IF Staining of HepG2 using CL488-68126

    IF Staining of HepG2 using CL488-68126

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 RBP5 antibody (CL488-68126, Clone: 1G7E2 ) at dilution of 1:200.

  • IF Staining of A431 using CL488-68126

    IF Staining of A431 using CL488-68126

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 RBP5 antibody (CL488-68126, Clone: 1G7E2 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated RBP5 Monoclonal antibody

RBP5 Monoclonal Antibody for IF/ICC
Cat No. CL488-68126
CloneNo. 1G7E2

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig

Applications

IF/ICC

Synonyms

CRBP III, CRBP3, CRBPIII, HRBPiso, RBP5, Retinol binding protein 5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells, A431 cells
For other applications, we recommend the unconjugated version of this antibody, 68126-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68126 targets RBP5 in IF/ICC applications and shows reactivity with Human, pig samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, pig
Immunogen RBP5 fusion protein Ag24562 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name retinol binding protein 5, cellular
Synonyms CRBP III, CRBP3, CRBPIII, HRBPiso, RBP5, Retinol binding protein 5
Calculated Molecular Weight 135 aa, 16 kDa
Observed Molecular Weight 16-18 kDa
GenBank Accession NumberBC029355
Gene Symbol RBP5
Gene ID (NCBI) 83758
RRIDAB_3084408
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP82980
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RBP5 (CRBP III) belongs to the fatty-acid binding protein (FABP) family. Human liver and kidney contain the highest levels of CRBP III mRNA, and CRBP III is as a major human intracellular carrier of retinol (PMID:11274389). RBP5 iron response are reversible by genetic complementation. And increased RBP5 expression occurs by a post-transcriptional feedback mechanism whereby RBP5 interacts with its own, and with PAP2 mRNAs. RBP5 is the most significant of the proteins upregulated by iron starvation (PMID:34161395). RBP5 has an estimated molecular mass (15.9 kDa) typical of this protein superfamily (PMID:11274389).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RBP5 antibody CL488-68126Download protocol
Standard Protocols
Click here to view our Standard Protocols