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验证数据展示

  • IF Staining of HeLa using CL488-66716

    IF Staining of HeLa using CL488-66716

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 RBX1 antibody (CL488-66716, Clone: 1F4D9 ) at dilution of 1:500, CL594-phalloidin (red).

CoraLite® Plus 488-conjugated RBX1 Monoclonal antibody

RBX1 Monoclonal Antibody for IF/ICC
Cat No. CL488-66716

产品说明书

CloneNo. 1F4D9

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF/ICC

别名

Protein ZYP, EC:2.3.2.27, E3 ubiquitin-protein transferase RBX1, E3 ubiquitin-protein ligase RBX1, N-terminally processed, E3 ubiquitin-protein ligase RBX1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RBX1 Ready-To-Use IHC Kit. RBX1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66716-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66716 targets RBX1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 RBX1 fusion protein Ag7005 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ring-box 1
别名 Protein ZYP, EC:2.3.2.27, E3 ubiquitin-protein transferase RBX1, E3 ubiquitin-protein ligase RBX1, N-terminally processed, E3 ubiquitin-protein ligase RBX1
计算分子量 12 kDa
观测分子量 12-15 kDa
GenBank蛋白编号BC001466
基因名称 RBX1
Gene ID (NCBI) 9978
RRIDAB_2919360
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP62877
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RBX1(RING-box protein 1) is also named as RNF75, ROC1 and is a requisite component of the multi- subunit SCF family E3s36-40. It mediates the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RBX1 antibody CL488-66716Download protocol
Standard Protocols
Click here to view our Standard Protocols