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验证数据展示

  • IF Staining of MCF-7 using CL488-66170

    IF Staining of MCF-7 using CL488-66170

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 RCAS1 antibody (CL488-66170, Clone: 4H8A12 ) at dilution of 1:200.

  • FC experiment of Jurkat using CL488-66170

    FC experiment of Jurkat using CL488-66170

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RCAS1 (CL488-66170, Clone:4H8A12) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated RCAS1 Monoclonal antibody

RCAS1 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-66170

产品说明书

CloneNo. 4H8A12

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

EBAG9, Estrogen receptor-binding fragment-associated gene 9 protein, EBAG 9, EB9, Cancer-associated surface antigen RCAS1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy RCAS1 Ready-To-Use IHC Kit. RCAS1 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66170 targets RCAS1 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 RCAS1 fusion protein Ag2905 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 estrogen receptor binding site associated, antigen, 9
别名 EBAG9, Estrogen receptor-binding fragment-associated gene 9 protein, EBAG 9, EB9, Cancer-associated surface antigen RCAS1
计算分子量 213 aa, 24 kDa
观测分子量 34 kDa
GenBank蛋白编号BC017729
基因名称 RCAS1
Gene ID (NCBI) 9166
RRIDAB_3084216
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO00559
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Estrogen receptor-binding fragment-associated antigen 9 (EBAG9) gene was identified as an estrogen-responsive gene. The gene product, receptor-binding cancer antigen expressed on SiSo cells (RCAS1), is associated with aggressive characteristics and poor overall survival for 15 different human malignancies. The correlation between RCAS1 expression and several clinicopathological variables, including tumor size, clinical stage, invasion depth and lymph node metastasis highlights this molecule's clinical significance. Expression of RCAS1 in tumor cells plays an important role in evasion from host immune system resulting tumor progression, invasion and metastasis. Further exploration of RCAS1 biological function will facilitate development of novel therapeutic strategies that target RCAS1.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RCAS1 antibody CL488-66170Download protocol
FC protocol for CL Plus 488 RCAS1 antibody CL488-66170Download protocol
Standard Protocols
Click here to view our Standard Protocols