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- KD/KO Validated
RCN2 Polyclonal antibody
RCN2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, mouse lung tissue, human brain tissue, HeLa cells |
Positive IP detected in | mouse lung tissue |
Positive IHC detected in | human gliomas tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells, HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:350-1:1400 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10193-2-AP targets RCN2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IHC, CoIP, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Immunogen | RCN2 fusion protein Ag0247 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | reticulocalbin 2, EF-hand calcium binding domain |
Synonyms | ERC 55, E6BP, E6-binding protein, E6 binding protein, Calcium-binding protein ERC-55 |
Calculated Molecular Weight | 37 kDa |
Observed Molecular Weight | 55 kDa |
GenBank Accession Number | BC004892 |
Gene Symbol | RCN2 |
Gene ID (NCBI) | 5955 |
RRID | AB_2180018 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q14257 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for RCN2 antibody 10193-2-AP | Download protocol |
IHC protocol for RCN2 antibody 10193-2-AP | Download protocol |
IF protocol for RCN2 antibody 10193-2-AP | Download protocol |
IP protocol for RCN2 antibody 10193-2-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Med Chemical corrector treatment ameliorates increased seizure susceptibility in a mouse model of familial epilepsy. | ||
Cell Metab A mechanosensitive lipolytic factor in the bone marrow promotes osteogenesis and lymphopoiesis. | ||
Proc Natl Acad Sci U S A Stk40 links the pluripotency factor Oct4 to the Erk/MAPK pathway and controls extraembryonic endoderm differentiation.
| ||
Elife Live-cell mapping of organelle-associated RNAs via proximity biotinylation combined with protein-RNA crosslinking. | ||
Elife Proteomic mapping of cytosol-facing outer mitochondrial and ER membranes in living human cells by proximity biotinylation. | ||
Am J Cancer Res YY1-mediated reticulocalbin-2 upregulation promotes the hepatocellular carcinoma progression via activating MYC signaling. |