验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy RCN2 Ready-To-Use IHC Kit. RCN2 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-10193 targets RCN2 in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | RCN2 fusion protein Ag0247 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | reticulocalbin 2, EF-hand calcium binding domain |
| Synonyms | ERC 55, E6BP, E6-binding protein, E6 binding protein, Calcium-binding protein ERC-55 |
| Calculated Molecular Weight | 37 kDa |
| Observed Molecular Weight | 55 kDa |
| GenBank Accession Number | BC004892 |
| Gene Symbol | RCN2 |
| Gene ID (NCBI) | 5955 |
| RRID | AB_3672432 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14257 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Reticulocalbin 2 (RCN2) is a Ca(2+)-binding protein that is specifically localized in the endoplasmic reticulum (ER). It is also termed ERC-55, i.e. ER calcium-binding protein of 55 kDa. It is a single copy gene and is encoded by an approximately 1900-base mRNA, which shows a ubiquitous expression pattern. The protein comprises an amino-terminal signal sequence followed by six copies of the EF-hand Ca2+ binding motif.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 RCN2 antibody CL488-10193 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |