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验证数据展示

  • IF Staining of HeLa using CL488-14643

    IF Staining of HeLa using CL488-14643

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 REEP5 antibody (CL488-14643) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of HeLa using CL488-14643

    FC experiment of HeLa using CL488-14643

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human REEP5 (CL488-14643) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated REEP5 Polyclonal antibody

REEP5 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-14643

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

TB2, Receptor expression-enhancing protein 5, Protein TB2, Polyposis locus protein 1, DP1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells, A549 cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 14643-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-14643 targets REEP5 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 REEP5 fusion protein Ag6229 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 receptor accessory protein 5
别名 TB2, Receptor expression-enhancing protein 5, Protein TB2, Polyposis locus protein 1, DP1
计算分子量 21 kDa
观测分子量 18-21 kDa
GenBank蛋白编号BC065926
基因名称 REEP5
Gene ID (NCBI) 7905
RRIDAB_2919098
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen Affinity Purified
UNIPROT IDQ00765
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

REEP5 (receptor expression-enhancing protein 5), also named C5orf18, DP1, or TB2, is a membrane protein belonging to the DP1/Yop1p protein family. All REEP proteins contain a conserved TB2/DP1, HVA22 domain which is involved in intracellular trafficking and secretion (PMID: 19123125). REEP5 is expressed in circumvallate papillae and testis and may have the function of influencing functional cell surface expression of taste receptors (PMID: 16720576). REEP5 can interact with atlastins (ATL1 and ATL2) which are involved in modulating the structure of the ER network (PMID: 19665976).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 REEP5 antibody CL488-14643Download protocol
Standard Protocols
Click here to view our Standard Protocols