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验证数据展示

  • WB analysis using 83305-6-RR

    WB analysis using 83305-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83305-6-RR (RELB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of Raji using 83305-6-RR

    FC experiment of Raji using 83305-6-RR

    1x10^6 Raji cells were intracellularly stained with 0.25 ug RELB Recombinant antibody (83305-6-RR, Clone:242336G4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83305-6-RR

    Affinity and Kinetic Characterization of 83305-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83305-6-RR against Human RELB were performed. The affinity constant is below 1 pM.

RELB Recombinant antibody

RELB Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83305-6-RR
CloneNo. 242336G4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

Transcription factor RelB, I-Rel, 242336G4

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inRaji cells, Daudi cells, MDA-MB-231 cells
Positive FC (Intra) detected inRaji cells
Planning an IHC experiment? We recommend our IHCeasy RELB Ready-To-Use IHC Kit. RELB primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83305-6-RR targets RELB in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen RELB fusion protein Ag13824 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name v-rel reticuloendotheliosis viral oncogene homolog B
Synonyms Transcription factor RelB, I-Rel, 242336G4
Calculated Molecular Weight 62 kDa
Observed Molecular Weight 62-65 kDa
GenBank Accession NumberBC028013
Gene Symbol RELB
Gene ID (NCBI) 5971
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ01201
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RELB, also named as Transcription factor RelB, is a 579 amino acid protein, which contains one RHD domain. RELB localizes in the nucleus and cytoplasm. RELB is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis.

实验方案

Product Specific Protocols
WB protocol for RELB antibody 83305-6-RRDownload protocol
FC protocol for RELB antibody 83305-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols