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验证数据展示

  • FC experiment of Jurkat using CL488-25027

    FC experiment of Jurkat using CL488-25027

    1X10^6 Jurkat cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human RELB (CL488-25027) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HeLa using CL488-25027

    IF Staining of HeLa using CL488-25027

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 RELB antibody (CL488-25027) at dilution of 1:200.

CoraLite® Plus 488-conjugated RELB Polyclonal antibody

RELB Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-25027

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

IF/ICC, FC (Intra) and More (1)

别名

Transcription factor RelB, I-Rel

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy RELB Ready-To-Use IHC Kit. RELB primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 25027-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

CL488-25027 targets RELB in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
文献引用应用IF
经测试反应性 human
文献引用反应性mouse
免疫原 RELB fusion protein Ag13824 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 v-rel reticuloendotheliosis viral oncogene homolog B
别名 Transcription factor RelB, I-Rel
计算分子量 62 kDa
观测分子量 62-65 kDa
GenBank蛋白编号BC028013
基因名称 RELB
Gene ID (NCBI) 5971
RRIDAB_2919201
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ01201
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RELB, also named as Transcription factor RelB, is a 579 amino acid protein, which contains one RHD domain. RELB localizes in the nucleus and cytoplasm. RELB is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RELB antibody CL488-25027Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Cytokine

Extracellular HSP70 facilitated β-glucan induced trained immunity in macrophages to suppress sepsis via TLR2-NF-κB axis

Authors - Ran Qi
View Article