RENALASE Monoclonal antibody
RENALASE Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human and More (1)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
3A12F5
验证数据展示
经过测试的应用
| Positive WB detected in | OS-RC-2 cells, A431 cells, MG-63 cells, ACHN cells, Caki-1 cells |
| Positive IHC detected in | human kidney tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
产品信息
60128-1-Ig targets RENALASE in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Immunogen | RENALASE fusion protein Ag13061 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | chromosome 10 open reading frame 59 |
| Synonyms | C10orf59, 3A12F5, Monoamine oxidase C, MAO-C, MAO C |
| Calculated Molecular Weight | 38 kDa |
| Observed Molecular Weight | 38 kDa |
| GenBank Accession Number | BC005364 |
| Gene Symbol | RENALASE |
| Gene ID (NCBI) | 55328 |
| RRID | AB_2034903 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q5VYX0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RNLS, also named as Renalase, C10orf59 and MAO-C, belongs to the renalase family. It is probable FAD-dependent amine oxidase secreted by the kidney, which circulates in blood and modulates cardiac function and systemic blood pressure. RNLS degrades catecholamines such as dopamine, norepinephrine and epinephrine in vitro. It lowers blood pressure in vivo by decreasing cardiac contractility and heart rate and preventing a compensatory increase in peripheral vascular tone, suggesting a causal link to the increased plasma catecholamine and heightened cardiovascular risk. High concentrations of catecholamines activate plasma renalase and promotes its secretion and synthesis. RNLS has physiologically relevant catecholamine-oxidizing activity. (PMID:15841207 ) This antibody is specific to RNLS.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RENALASE antibody 60128-1-Ig | Download protocol |
| IHC protocol for RENALASE antibody 60128-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Am Soc Hypertens Human renalase: a review of its biology, function, and implications for hypertension. | ||
bioRxiv Renalase inhibition regulates β cell metabolism to defend against acute and chronic stress |
