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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 84846-4-RR (same clone as 84846-4-PBS)

    WB analysis using 84846-4-RR (same clone as 84846-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84846-4-RR (REST antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84846-4-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84846-4-RR (same clone as 84846-4-PBS)

    IF Staining of HeLa using 84846-4-RR (same clone as 84846-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using REST antibody (84846-4-RR, Clone: 241789D3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84846-4-PBS in a different storage buffer formulation.

REST Recombinant antibody, PBS Only

REST Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 84846-4-PBS

产品说明书

CloneNo. 241789D3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

XBR, X2 box repressor, NRSF, 241789D3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy REST Ready-To-Use IHC Kit. REST primary antibody included.

产品信息

84846-4-PBS targets REST in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 REST fusion protein Ag17707 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RE1-silencing transcription factor
别名 XBR, X2 box repressor, NRSF, 241789D3
计算分子量 1097 aa, 122 kDa
观测分子量 200 kDa
GenBank蛋白编号BC132859
基因名称 REST
Gene ID (NCBI) 5978
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ13127
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

REST also named as NRSF or X2 box repressor is a 1097 amino acid protein, which contains 9 C2H2-type zinc fingers. REST as a transcriptional repressor binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Repressor element 1 (RE1) silencing transcription factor (REST), is widely expressed during embryogenesis and plays a strategic role in terminal neuronal differentiation. The molecular weight of REST is 121 kDa, but REST was detected at 200 kDa by western blot in our result, which is similar to the article (PMID: 16764822).