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验证数据展示

  • WB analysis using 84846-4-RR

    WB analysis using 84846-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84846-4-RR (REST antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 84846-4-RR

    IF Staining of HeLa using 84846-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using REST antibody (84846-4-RR, Clone: 241789D3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

REST Recombinant antibody

REST Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84846-4-RR

产品说明书

CloneNo. 241789D3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

XBR, X2 box repressor, NRSF, 241789D3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, K-562 cells, BxPC-3 cells, SW 1990 cells, L02 cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy REST Ready-To-Use IHC Kit. REST primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84846-4-RR targets REST in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 REST fusion protein Ag17707 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RE1-silencing transcription factor
别名 XBR, X2 box repressor, NRSF, 241789D3
计算分子量 1097 aa, 122 kDa
观测分子量 200 kDa
GenBank蛋白编号BC132859
基因名称 REST
Gene ID (NCBI) 5978
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ13127
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

REST also named as NRSF or X2 box repressor is a 1097 amino acid protein, which contains 9 C2H2-type zinc fingers. REST as a transcriptional repressor binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Repressor element 1 (RE1) silencing transcription factor (REST), is widely expressed during embryogenesis and plays a strategic role in terminal neuronal differentiation. The molecular weight of REST is 121 kDa, but REST was detected at 200 kDa by western blot in our result, which is similar to the article (PMID: 16764822).

实验方案

Product Specific Protocols
WB protocol for REST antibody 84846-4-RRDownload protocol
IF protocol for REST antibody 84846-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols