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验证数据展示

  • IF Staining of A549 using CL488-22242

    IF Staining of A549 using CL488-22242

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 REST antibody (CL488-22242) at dilution of 1:200.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated REST Polyclonal antibody

REST Polyclonal Antibody for IF/ICC
Cat No. CL488-22242

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

IF/ICC

Synonyms

NRSF, REST, X2 box repressor, XBR

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy REST Ready-To-Use IHC Kit. REST primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 22242-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-22242 targets REST in IF/ICC applications and shows reactivity with human, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, rat
Immunogen REST fusion protein Ag17707 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RE1-silencing transcription factor
Synonyms NRSF, REST, X2 box repressor, XBR
Calculated Molecular Weight 1097 aa, 122 kDa
Observed Molecular Weight 200 kDa
GenBank Accession NumberBC132859
Gene Symbol REST
Gene ID (NCBI) 5978
RRIDAB_3084065
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13127
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

REST also named as NRSF or X2 box repressor is a 1097 amino acid protein, which contains 9 C2H2-type zinc fingers. REST as transcriptional repressor binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Repressor element 1 (RE1) silencing transcription factor (REST), is widely expressed during embryogenesis, and plays a strategic role in terminal neuronal differentiation. The molecular weight of REST is 121 kDa, but REST was detected 200kDa by western blot in our result, which is similar to the one article (PMID: 16764822).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 REST antibody CL488-22242Download protocol
Standard Protocols
Click here to view our Standard Protocols