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验证数据展示

  • IF Staining of A549 using CL488-22242

    IF Staining of A549 using CL488-22242

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 REST antibody (CL488-22242) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated REST Polyclonal antibody

REST Polyclonal Antibody for IF/ICC
Cat No. CL488-22242

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

IF/ICC

别名

NRSF, REST, X2 box repressor, XBR

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy REST Ready-To-Use IHC Kit. REST primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 22242-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-22242 targets REST in IF/ICC applications and shows reactivity with human, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, rat
免疫原 REST fusion protein Ag17707 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RE1-silencing transcription factor
别名 NRSF, REST, X2 box repressor, XBR
计算分子量 1097 aa, 122 kDa
观测分子量 200 kDa
GenBank蛋白编号BC132859
基因名称 REST
Gene ID (NCBI) 5978
RRIDAB_3084065
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13127
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

REST also named as NRSF or X2 box repressor is a 1097 amino acid protein, which contains 9 C2H2-type zinc fingers. REST as transcriptional repressor binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Repressor element 1 (RE1) silencing transcription factor (REST), is widely expressed during embryogenesis, and plays a strategic role in terminal neuronal differentiation. The molecular weight of REST is 121 kDa, but REST was detected 200kDa by western blot in our result, which is similar to the one article (PMID: 16764822).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 REST antibody CL488-22242Download protocol
Standard Protocols
Click here to view our Standard Protocols