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  • Featured Product
  • KD/KO Validated

REXO4 Polyclonal antibody

REXO4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 18890-1-AP

Print datasheet

Synonyms

XPMC2, REX4, REX 4, PMC2, hPMC2

验证数据展示

  • WB analysis using 18890-1-AP

    WB analysis using 18890-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18890-1-AP (REXO4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 18890-1-AP

    IHC staining of human testis using 18890-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 18890-1-AP (REXO4 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human testis using 18890-1-AP

    IHC staining of human testis using 18890-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 18890-1-AP (REXO4 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HeLa using 18890-1-AP

    IF Staining of HeLa using 18890-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using REXO4 antibody (18890-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18890-1-AP targets REXO4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen REXO4 fusion protein Ag13473 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name REX4, RNA exonuclease 4 homolog (S. cerevisiae)
Synonyms XPMC2, REX4, REX 4, PMC2, hPMC2
Calculated Molecular Weight 422 aa, 47 kDa
Observed Molecular Weight 47-50 kDa
GenBank Accession NumberBC009274
Gene Symbol REXO4
Gene ID (NCBI) 57109
RRIDAB_10643245
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9GZR2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for REXO4 antibody 18890-1-APDownload protocol
IHC protocol for REXO4 antibody 18890-1-APDownload protocol
IF protocol for REXO4 antibody 18890-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

J Gastrointest Oncol

REXO4 acts as a biomarker and promotes hepatocellular carcinoma progression.

Authors - Yun Ruan
  • KD Validated
View Article
humanWB

J Adv Res

PROTAC derivatization of natural products for target identification and drug discovery: Design of evodiamine-based PROTACs as novel REXO4 degraders

Authors - Shuqiang Chen
View Article