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验证数据展示

  • WB analysis of HeLa using 85148-1-RR

    WB analysis of HeLa using 85148-1-RR

    HeLa cells were subjected to SDS PAGE followed by western blot with 85148-1-RR (RFC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 85148-1-RR

    Affinity and Kinetic Characterization of 85148-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85148-1-RR against Human RFC1 were performed. The affinity constant is below 1 pM.

RFC1 Recombinant antibody

RFC1 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85148-1-RR

产品说明书

CloneNo. 242818A5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

242818A5, A1, A1 140 kDa subunit, Activator 1 140 kDa subunit, Activator 1 large subunit

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, HEK-293T cells, Jurkat cells, A431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85148-1-RR targets RFC1 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 RFC1 fusion protein Ag13585 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 replication factor C (activator 1) 1, 145kDa
别名 242818A5, A1, A1 140 kDa subunit, Activator 1 140 kDa subunit, Activator 1 large subunit
计算分子量 128 kDa
观测分子量128-150 kDa
GenBank蛋白编号BC051751
基因名称 RFC1
Gene ID (NCBI) 5981
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP35251
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Replication factor C subunit 1 (RFC1) is a pentameric DNA polymerase accessory protein involved in DNA replication and repair (PMID: 10051561). It was reported as a cause of cerebellar ataxia with neuropathy and vestibular areflexia syndrome (CANVAS) (PMID: 30926972).

实验方案

Product Specific Protocols
WB protocol for RFC1 antibody 85148-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols