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验证数据展示

  • WB analysis using 68290-1-Ig

    WB analysis using 68290-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68290-1-Ig (RFC3 antibody) at dilution of 1:10000 and incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of NIH/3T3 using 68290-1-Ig

    WB analysis of NIH/3T3 using 68290-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68290-1-Ig (RFC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HSC-T6 using 68290-1-Ig

    WB analysis of HSC-T6 using 68290-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68290-1-Ig (RFC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

RFC3 Monoclonal antibody

RFC3 Monoclonal Antibody for WB, ELISA
Cat No. 68290-1-Ig

Print datasheet

CloneNo. 2G3A8

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, ELISA

Synonyms

A1 38 kDa subunit, Activator 1 38 kDa subunit, Activator 1 subunit 3, Replication factor C subunit 3, RF C 38 kDa subunit, RFC3, RFC38

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, HSC-T6 cells, NIH/3T3 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68290-1-Ig targets RFC3 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen RFC3 fusion protein Ag28365 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name replication factor C (activator 1) 3, 38kDa
Synonyms A1 38 kDa subunit, Activator 1 38 kDa subunit, Activator 1 subunit 3, Replication factor C subunit 3, RF C 38 kDa subunit, RFC3, RFC38
Calculated Molecular Weight 40 aa, 6 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC000149
Gene Symbol RFC3
Gene ID (NCBI) 5983
RRIDAB_2935370
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP40938
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RFC3, also known as RFC38, is a 356 amino acid protein, which localizes in the nucleus. Replication factor C (RFC) is a component of the eukaryotic DNA polymerase. In all eukaryotic cell cycles, it is essential for DNA duplication, DNA injury repair, and checkpoint control. RFC consists of five subunits (RFC1-5). The interrelationships between RFC2, RFC3, and the c-MYC oncogene (transcription factor) can induce cell division and proliferation. RFC3 is associated with liver, breast, esophageal and ovarian cancer, and serves a critical role in cellular proliferation, invasion, and metastasis. RFC3 exists in two isoforms with molecular weights of 34 kDa and 38-40 kDa.

实验方案

Product Specific Protocols
WB protocol for RFC3 antibody 68290-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols