验证数据展示
经过测试的应用
Positive WB detected in | LNCaP cells, HSC-T6 cells, NIH/3T3 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
68290-1-Ig targets RFC3 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | Human, Mouse, Rat |
免疫原 | RFC3 fusion protein Ag28365 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | replication factor C (activator 1) 3, 38kDa |
别名 | A1 38 kDa subunit, Activator 1 38 kDa subunit, Activator 1 subunit 3, Replication factor C subunit 3, RF C 38 kDa subunit, RFC3, RFC38 |
计算分子量 | 40 aa, 6 kDa |
观测分子量 | 38 kDa |
GenBank蛋白编号 | BC000149 |
基因名称 | RFC3 |
Gene ID (NCBI) | 5983 |
RRID | AB_2935370 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | P40938 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RFC3, also known as RFC38, is a 356 amino acid protein, which localizes in the nucleus. Replication factor C (RFC) is a component of the eukaryotic DNA polymerase. In all eukaryotic cell cycles, it is essential for DNA duplication, DNA injury repair, and checkpoint control. RFC consists of five subunits (RFC1-5). The interrelationships between RFC2, RFC3, and the c-MYC oncogene (transcription factor) can induce cell division and proliferation. RFC3 is associated with liver, breast, esophageal and ovarian cancer, and serves a critical role in cellular proliferation, invasion, and metastasis. RFC3 exists in two isoforms with molecular weights of 34 kDa and 38-40 kDa.
实验方案
Product Specific Protocols | |
---|---|
WB protocol for RFC3 antibody 68290-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |