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RFC3 Monoclonal antibody, PBS Only

RFC3 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G3A8

Cat no : 68290-1-PBS

Print datasheet

Synonyms

A1 38 kDa subunit, Activator 1 38 kDa subunit, Activator 1 subunit 3, Replication factor C subunit 3, RF C 38 kDa subunit, RFC3, RFC38

验证数据展示

  • WB analysis using 68290-1-Ig (same clone as 68290-1-PBS)

    WB analysis using 68290-1-Ig (same clone as 68290-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68290-1-Ig (RFC3 antibody) at dilution of 1:10000 and incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68290-1-PBS in a different storage buffer formulation.

  • WB analysis of NIH/3T3 using 68290-1-Ig (same clone as 68290-1-PBS)

    WB analysis of NIH/3T3 using 68290-1-Ig (same clone as 68290-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68290-1-Ig (RFC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68290-1-PBS in a different storage buffer formulation.

  • WB analysis of HSC-T6 using 68290-1-Ig (same clone as 68290-1-PBS)

    WB analysis of HSC-T6 using 68290-1-Ig (same clone as 68290-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68290-1-Ig (RFC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68290-1-PBS in a different storage buffer formulation.

产品信息

68290-1-PBS targets RFC3 in WB, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen RFC3 fusion protein Ag28365 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name replication factor C (activator 1) 3, 38kDa
Synonyms A1 38 kDa subunit, Activator 1 38 kDa subunit, Activator 1 subunit 3, Replication factor C subunit 3, RF C 38 kDa subunit, RFC3, RFC38
Calculated Molecular Weight 40 aa, 6 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC000149
Gene Symbol RFC3
Gene ID (NCBI) 5983
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP40938
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RFC3, also known as RFC38, is a 356 amino acid protein, which localizes in the nucleus. Replication factor C (RFC) is a component of the eukaryotic DNA polymerase. In all eukaryotic cell cycles, it is essential for DNA duplication, DNA injury repair, and checkpoint control. RFC consists of five subunits (RFC1-5). The interrelationships between RFC2, RFC3, and the c-MYC oncogene (transcription factor) can induce cell division and proliferation. RFC3 is associated with liver, breast, esophageal and ovarian cancer, and serves a critical role in cellular proliferation, invasion, and metastasis. RFC3 exists in two isoforms with molecular weights of 34 kDa and 38-40 kDa.