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×
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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 68290-1-Ig (same clone as 68290-1-PBS)

    WB analysis using 68290-1-Ig (same clone as 68290-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68290-1-Ig (RFC3 antibody) at dilution of 1:10000 and incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68290-1-PBS in a different storage buffer formulation.

  • WB analysis of NIH/3T3 using 68290-1-Ig (same clone as 68290-1-PBS)

    WB analysis of NIH/3T3 using 68290-1-Ig (same clone as 68290-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68290-1-Ig (RFC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68290-1-PBS in a different storage buffer formulation.

  • WB analysis of HSC-T6 using 68290-1-Ig (same clone as 68290-1-PBS)

    WB analysis of HSC-T6 using 68290-1-Ig (same clone as 68290-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68290-1-Ig (RFC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68290-1-PBS in a different storage buffer formulation.

RFC3 Monoclonal antibody, PBS Only

RFC3 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68290-1-PBS

产品说明书

CloneNo. 2G3A8

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat

应用

WB, Indirect ELISA

别名

A1 38 kDa subunit, Activator 1 38 kDa subunit, Activator 1 subunit 3, Replication factor C subunit 3, RF C 38 kDa subunit, RFC3, RFC38

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68290-1-PBS targets RFC3 in WB, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原 RFC3 fusion protein Ag28365 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 replication factor C (activator 1) 3, 38kDa
别名 A1 38 kDa subunit, Activator 1 38 kDa subunit, Activator 1 subunit 3, Replication factor C subunit 3, RF C 38 kDa subunit, RFC3, RFC38
计算分子量 40 aa, 6 kDa
观测分子量 38 kDa
GenBank蛋白编号BC000149
基因名称 RFC3
Gene ID (NCBI) 5983
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP40938
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RFC3, also known as RFC38, is a 356 amino acid protein, which localizes in the nucleus. Replication factor C (RFC) is a component of the eukaryotic DNA polymerase. In all eukaryotic cell cycles, it is essential for DNA duplication, DNA injury repair, and checkpoint control. RFC consists of five subunits (RFC1-5). The interrelationships between RFC2, RFC3, and the c-MYC oncogene (transcription factor) can induce cell division and proliferation. RFC3 is associated with liver, breast, esophageal and ovarian cancer, and serves a critical role in cellular proliferation, invasion, and metastasis. RFC3 exists in two isoforms with molecular weights of 34 kDa and 38-40 kDa.