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验证数据展示

  • WB analysis using 68390-1-Ig

    WB analysis using 68390-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68390-1-Ig (RFC4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • FC experiment of HepG2 using 68390-1-Ig

    FC experiment of HepG2 using 68390-1-Ig

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug RFC4 Monoclonal antibody (68390-1-Ig, Clone:1A6A11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

RFC4 Monoclonal antibody

RFC4 Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 68390-1-Ig

产品说明书

CloneNo. 1A6A11

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, FC (Intra), ELISA

别名

RFC37, RF-C 37 kDa subunit, A1, 1A6A11

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inU2OS cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, 4T1 cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68390-1-Ig targets RFC4 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RFC4 fusion protein Ag33295 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 replication factor C (activator 1) 4, 37kDa
别名 RFC37, RF-C 37 kDa subunit, A1, 1A6A11
计算分子量 37 kDa
观测分子量 37-40 kDa
GenBank蛋白编号BC017452
基因名称 RFC4
Gene ID (NCBI) 5984
RRIDAB_3085108
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP35249
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RFC4 antibody 68390-1-IgDownload protocol
FC protocol for RFC4 antibody 68390-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols