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RFC4 Monoclonal antibody

RFC4 Monoclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1A6A11

Cat no : 68390-1-Ig

Print datasheet

Synonyms

RFC37, RF-C 37 kDa subunit, A1, 1A6A11

验证数据展示

  • WB analysis using 68390-1-Ig

    WB analysis using 68390-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68390-1-Ig (RFC4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • FC experiment of HepG2 using 68390-1-Ig

    FC experiment of HepG2 using 68390-1-Ig

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug RFC4 Monoclonal antibody (68390-1-Ig, Clone:1A6A11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive WB detected inU2OS cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, 4T1 cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68390-1-Ig targets RFC4 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen RFC4 fusion protein Ag33295 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name replication factor C (activator 1) 4, 37kDa
Synonyms RFC37, RF-C 37 kDa subunit, A1, 1A6A11
Calculated Molecular Weight 37 kDa
Observed Molecular Weight 37-40 kDa
GenBank Accession NumberBC017452
Gene Symbol RFC4
Gene ID (NCBI) 5984
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP35249
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RFC4 antibody 68390-1-IgDownload protocol
FC protocol for RFC4 antibody 68390-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols