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Conjugate
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RFX1 Monoclonal antibody, PBS Only

RFX1 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G10H6

Cat no : 68057-1-PBS

Print datasheet

Synonyms

RFX, EF-C, EF C, 1G10H6

验证数据展示

  • WB analysis using 68057-1-Ig (same clone as 68057-1-PBS)

    WB analysis using 68057-1-Ig (same clone as 68057-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68057-1-Ig (RFX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 68057-1-PBS in a different storage buffer formulation.

产品信息

68057-1-PBS targets RFX1 in WB, Indirect ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity Human, Mouse
Immunogen RFX1 fusion protein Ag25442 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name regulatory factor X, 1 (influences HLA class II expression)
Synonyms RFX, EF-C, EF C, 1G10H6
Calculated Molecular Weight 105 kDa
Observed Molecular Weight135 kDa
GenBank Accession NumberBC049826
Gene Symbol RFX1
Gene ID (NCBI) 5989
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP22670
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RFX1, also named as MHC class II regulatory factor RFX1, is a 979 amino acid protein which contains 1 RFX-type winged-helix DNA-binding domain and belongs to the RFX family. RFX1 can binds DNA as a homodimer and heterodimerizes with RFX2 and RFX3. RFX1 as a regulatory factor is essential for MHC class II genes expression. RFX1 protein is modified after translation and its molecular weight could be migrate to 130-140 kDa (PMID: 20189986 ).