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验证数据展示

  • WB analysis using 68057-1-Ig

    WB analysis using 68057-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68057-1-Ig (RFX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control.

RFX1 Monoclonal antibody

RFX1 Monoclonal Antibody for WB, ELISA
Cat No. 68057-1-Ig

产品说明书

CloneNo. 1G10H6

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse

应用

WB, ELISA

别名

EF C, Enhancer factor C, Regulatory factor X 1, RFX, RFX1, Transcription factor RFX1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, NIH/3T3 cells, U2OS cells, K-562 cells, A549 cells, MCF-7 cells, LnCaP cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68057-1-Ig targets RFX1 in WB, ELISA applications and shows reactivity with Human, Mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, Mouse
免疫原 RFX1 fusion protein Ag25442 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 regulatory factor X, 1 (influences HLA class II expression)
别名 EF C, Enhancer factor C, Regulatory factor X 1, RFX, RFX1, Transcription factor RFX1
计算分子量 105 kDa
观测分子量135 kDa
GenBank蛋白编号BC049826
基因名称 RFX1
Gene ID (NCBI) 5989
RRIDAB_2918798
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP22670
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RFX1, also named as MHC class II regulatory factor RFX1, is a 979 amino acid protein which contains 1 RFX-type winged-helix DNA-binding domain and belongs to the RFX family. RFX1 can binds DNA as a homodimer and heterodimerizes with RFX2 and RFX3. RFX1 as a regulatory factor is essential for MHC class II genes expression. RFX1 protein is modified after translation and its molecular weight could be migrate to 130-140 kDa (PMID: 20189986 ).

实验方案

Product Specific Protocols
WB protocol for RFX1 antibody 68057-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols