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CoraLite® Plus 488-conjugated RFX1 Monoclonal antibody

RFX1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G10H6

Cat no : CL488-68057

Print datasheet

Synonyms

RFX, EF-C, EF C, 1G10H6

验证数据展示

  • FC experiment of Jurkat using CL488-68057

    FC experiment of Jurkat using CL488-68057

    1X10^6 Jurkat cells were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human RFX1 (CL488-68057, Clone:1G10H6) (red), or 0.2 ug x. Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

经过测试的应用

Positive FC (Intra) detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 68057-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68057 targets RFX1 in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen RFX1 fusion protein Ag25442 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name regulatory factor X, 1 (influences HLA class II expression)
Synonyms RFX, EF-C, EF C, 1G10H6
Calculated Molecular Weight 105 kDa
Observed Molecular Weight135 kDa
GenBank Accession NumberBC049826
Gene Symbol RFX1
Gene ID (NCBI) 5989
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP22670
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RFX1, also named as MHC class II regulatory factor RFX1, is a 979 amino acid protein which contains 1 RFX-type winged-helix DNA-binding domain and belongs to the RFX family. RFX1 can binds DNA as a homodimer and heterodimerizes with RFX2 and RFX3. RFX1 as a regulatory factor is essential for MHC class II genes expression. RFX1 protein is modified after translation and its molecular weight could be migrate to 130-140 kDa (PMID: 20189986 ).