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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 17947-1-AP (same clone as 17947-1-PBS)

    WB analysis using 17947-1-AP (same clone as 17947-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17947-1-AP (RGN/SMP30 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17947-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse liver using 17947-1-AP (same clone as 17947-1-PBS)

    IP experiment of mouse liver using 17947-1-AP (same clone as 17947-1-PBS)

    IP result of anti-RGN/SMP30 (IP:17947-1-AP, 4ug; Detection:17947-1-AP 1:15000) with mouse liver tissue lysate 3040 ug. This data was developed using the same antibody clone with 17947-1-PBS in a different storage buffer formulation.

  • IHC staining of rat kidney using 17947-1-AP (same clone as 17947-1-PBS)

    IHC staining of rat kidney using 17947-1-AP (same clone as 17947-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 17947-1-AP (RGN/SMP30 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17947-1-PBS in a different storage buffer formulation.

  • IHC staining of rat kidney using 17947-1-AP (same clone as 17947-1-PBS)

    IHC staining of rat kidney using 17947-1-AP (same clone as 17947-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 17947-1-AP (RGN/SMP30 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17947-1-PBS in a different storage buffer formulation.

RGN/SMP30 Polyclonal antibody, PBS Only

RGN/SMP30 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 17947-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

17947-1-PBS targets RGN/SMP30 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RGN/SMP30 fusion protein Ag12423 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 regucalcin (senescence marker protein-30)
别名
计算分子量 299 aa, 33 kDa
观测分子量 33 kDa, 25 kDa
GenBank蛋白编号BC073173
基因名称 RGN/SMP30
Gene ID (NCBI) 9104
RRIDAB_10644486
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15493
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Regucalcin (RGN), also known as SMP30, is a highly conserved, calcium-binding protein, that is preferentially expressed in the liver and kidney. SMP30 is preferentially localized in hepatocytes and renal proximal tubular epithelial cells. It is known to be related to aging, hepatocyte proliferation and tumorigenesis. It may be useful for HCC serologic screening, especially for the patients with AFP Negative. RGN encodes two isoforms: 33 kDa and 25 kDa.