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RGN/SMP30 Polyclonal antibody, PBS Only

RGN/SMP30 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 17947-1-PBS

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Synonyms

验证数据展示

  • WB analysis using 17947-1-AP (same clone as 17947-1-PBS)

    WB analysis using 17947-1-AP (same clone as 17947-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17947-1-AP (RGN/SMP30 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17947-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse liver using 17947-1-AP (same clone as 17947-1-PBS)

    IP experiment of mouse liver using 17947-1-AP (same clone as 17947-1-PBS)

    IP result of anti-RGN/SMP30 (IP:17947-1-AP, 4ug; Detection:17947-1-AP 1:15000) with mouse liver tissue lysate 3040 ug. This data was developed using the same antibody clone with 17947-1-PBS in a different storage buffer formulation.

  • IHC staining of rat kidney using 17947-1-AP (same clone as 17947-1-PBS)

    IHC staining of rat kidney using 17947-1-AP (same clone as 17947-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 17947-1-AP (RGN/SMP30 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17947-1-PBS in a different storage buffer formulation.

  • IHC staining of rat kidney using 17947-1-AP (same clone as 17947-1-PBS)

    IHC staining of rat kidney using 17947-1-AP (same clone as 17947-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 17947-1-AP (RGN/SMP30 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17947-1-PBS in a different storage buffer formulation.

产品信息

17947-1-PBS targets RGN/SMP30 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen RGN/SMP30 fusion protein Ag12423 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name regucalcin (senescence marker protein-30)
Synonyms
Calculated Molecular Weight 299 aa, 33 kDa
Observed Molecular Weight 33 kDa, 25 kDa
GenBank Accession NumberBC073173
Gene Symbol RGN/SMP30
Gene ID (NCBI) 9104
RRIDAB_10644486
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15493
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Regucalcin (RGN), also known as SMP30, is a highly conserved, calcium-binding protein, that is preferentially expressed in the liver and kidney. SMP30 is preferentially localized in hepatocytes and renal proximal tubular epithelial cells. It is known to be related to aging, hepatocyte proliferation and tumorigenesis. It may be useful for HCC serologic screening, especially for the patients with AFP Negative. RGN encodes two isoforms: 33 kDa and 25 kDa.