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RGNEF Recombinant antibody, PBS Only (Capture)

RGNEF Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241975E4

Cat no : 84570-2-PBS

Print datasheet

Synonyms

ARHGEF28, p190-RhoGEF, p190RhoGEF, p190 RhoGEF, ARG28

验证数据展示

  • WB analysis using 84570-2-RR (same clone as 84570-2-PBS)

    WB analysis using 84570-2-RR (same clone as 84570-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84570-2-RR (RGNEF antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84570-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01414-1

    Cytometric bead array standard curve of MP01414-1

    Cytometric bead array standard curve of MP01414-1, RGNEF Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84570-2-PBS. Detection antibody: 84570-1-PBS. Standard: Ag10915. Range: 0.469-60 ng/mL.

产品信息

84570-2-PBS targets RGNEF as part of a matched antibody pair:

MP01414-1: 84570-2-PBS capture and 84570-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen RGNEF fusion protein Ag10915 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name Rho-guanine nucleotide exchange factor
Synonyms ARHGEF28, p190-RhoGEF, p190RhoGEF, p190 RhoGEF, ARG28
Calculated Molecular Weight 192 kDa
Observed Molecular Weight192 kDa
GenBank Accession NumberBC012946
Gene Symbol RGNEF
Gene ID (NCBI) 64283
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8N1W1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ARHGEF28, also known as RGNEF or p190RhoGEF, is a 190 kDa RNA binding protein (RBP) that contains a Dbl/PH domain capable of RhoA activation. ARHGEF28 interacts with low molecular weight neurofilament mRNA and may be involved in the formation of amyotrophic lateral sclerosis neurofilament aggregates.