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验证数据展示

  • WB analysis using 84570-2-RR

    WB analysis using 84570-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84570-2-RR (RGNEF antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84570-2-RR

    Affinity and Kinetic Characterization of 84570-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84570-2-RR against Human RGNEF were performed. The affinity constant is below 1 pM.

RGNEF Recombinant antibody

RGNEF Recombinant Antibody for WB, ELISA
Cat No. 84570-2-RR

产品说明书

CloneNo. 241975E4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

ARHGEF28, p190-RhoGEF, p190RhoGEF, p190 RhoGEF, ARG28

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84570-2-RR targets RGNEF in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 RGNEF fusion protein Ag10915 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Rho-guanine nucleotide exchange factor
别名 ARHGEF28, p190-RhoGEF, p190RhoGEF, p190 RhoGEF, ARG28
计算分子量 192 kDa
观测分子量192 kDa
GenBank蛋白编号BC012946
基因名称 RGNEF
Gene ID (NCBI) 64283
RRIDAB_3672074
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8N1W1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARHGEF28, also known as RGNEF or p190RhoGEF, is a 190 kDa RNA binding protein (RBP) that contains a Dbl/PH domain capable of RhoA activation. ARHGEF28 interacts with low molecular weight neurofilament mRNA and may be involved in the formation of amyotrophic lateral sclerosis neurofilament aggregates.

实验方案

Product Specific Protocols
WB protocol for RGNEF antibody 84570-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols