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RGS12 Polyclonal antibody

RGS12 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29803-1-AP

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 29803-1-AP

    WB analysis using 29803-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29803-1-AP (RGS12 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of PC-3 using 29803-1-AP

    IF Staining of PC-3 using 29803-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using RGS12 antibody (29803-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 29803-1-AP

    WB analysis using 29803-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29803-1-AP (RGS12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat cerebellum using 29803-1-AP

    IHC staining of rat cerebellum using 29803-1-AP

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 29803-1-AP (RGS12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RGS12 antibody included

经过测试的应用

Positive WB detected inHepG2 cells, A431 cells, SH-SY5Y cells, THP-1 cells, NIH/3T3 cells
Positive IHC detected inrat cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy RGS12 Ready-To-Use IHC Kit. RGS12 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29803-1-AP targets RGS12 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen RGS12 fusion protein Ag30731 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name regulator of G-protein signaling 12
Synonyms
Calculated Molecular Weight 156 kDa
Observed Molecular Weight140-150 kDa
GenBank Accession NumberNM_198229
Gene Symbol RGS12
Gene ID (NCBI) 6002
RRIDAB_2935482
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14924
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Regulator of G-protein signaling 12 (RGS12) regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form. This antibody can be detected as 140-150 kDa.

实验方案

Product Specific Protocols
WB protocol for RGS12 antibody 29803-1-APDownload protocol
IHC protocol for RGS12 antibody 29803-1-APDownload protocol
IF protocol for RGS12 antibody 29803-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols