• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 85057-2-RR (same clone as 85057-2-PBS)

    WB analysis using 85057-2-RR (same clone as 85057-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85057-2-RR (RGS12 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85057-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01790-2

    Sandwich ELISA standard curve of MP01790-2

    Sandwich ELISA standard curve of MP01790-2, Human RGS12 Recombinant Matched Antibody Pair - PBS only. 85057-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag30731. 85057-2-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

  • Sample test of MP01790-2

    Sample test of MP01790-2

    The mean RGS12 concentration was determined to be 18,450.2 pg/mL in A431 cell extract based on a 1.3 mg/mL extract load, 6,327.2 pg/mL in HepG2 cell extract based on a 1.0 mg/mL extract load and 42,390.5 pg/mL in SH-SY5Y cell extract based on a 3.2 mg/mL extract load.

  • IF Staining of PC-3 using 85057-2-RR (same clone as 85057-2-PBS)

    IF Staining of PC-3 using 85057-2-RR (same clone as 85057-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using RGS12 antibody (85057-2-RR, Clone: 242530G11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85057-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01790-2

    Cytometric bead array standard curve of MP01790-2

    Cytometric bead array standard curve of MP01790-2, RGS12 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85057-3-PBS. Detection antibody: 85057-2-PBS. Standard: Ag30731. Range: 0.625-40 ng/mL.

  • Cytometric bead array standard curve of MP01790-1

    Cytometric bead array standard curve of MP01790-1

    Cytometric bead array standard curve of MP01790-1, RGS12 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85057-1-PBS. Detection antibody: 85057-2-PBS. Standard: Ag30731. Range: 0.625-40 ng/mL.

  • Affinity and Kinetic Characterization of 85057-2-RR

    Affinity and Kinetic Characterization of 85057-2-RR

    Biolayer interferometry (BLl) kinetic assays of 85057-2-RR against Human RGS12 were performed. The affinity constant is 0.611 nM.

RGS12 Recombinant antibody, PBS Only (Detector)

RGS12 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 85057-2-PBS
CloneNo. 242530G11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

242530G11

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy RGS12 Ready-To-Use IHC Kit. RGS12 primary antibody included.

产品信息

85057-2-PBS targets RGS12 as part of a matched antibody pair:

MP01790-1: 85057-1-PBS capture and 85057-2-PBS detection (validated in Cytometric bead array)

MP01790-2: 85057-3-PBS capture and 85057-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human, mouse, rat
Immunogen RGS12 fusion protein Ag30731 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name regulator of G-protein signaling 12
Synonyms 242530G11
Calculated Molecular Weight 156 kDa
Observed Molecular Weight140-150 kDa
GenBank Accession NumberNM_198229
Gene Symbol RGS12
Gene ID (NCBI) 6002
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14924
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C