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验证数据展示

  • WB analysis using 85057-2-RR

    WB analysis using 85057-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85057-2-RR (RGS12 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of PC-3 using 85057-2-RR

    IF Staining of PC-3 using 85057-2-RR

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using RGS12 antibody (85057-2-RR, Clone: 242530G11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 85057-2-RR

    Affinity and Kinetic Characterization of 85057-2-RR

    Biolayer interferometry (BLl) kinetic assays of 85057-2-RR against Human RGS12 were performed. The affinity constant is 0.611 nM.

RGS12 Recombinant antibody

RGS12 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85057-2-RR

产品说明书

CloneNo. 242530G11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

242530G11

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, HepG2 cells, SH-SY5Y cells, THP-1 cells, NIH/3T3 cells, C6 cells, mouse brain tissue
Positive IF/ICC detected inPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy RGS12 Ready-To-Use IHC Kit. RGS12 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85057-2-RR targets RGS12 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RGS12 fusion protein Ag30731 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 regulator of G-protein signaling 12
别名 242530G11
计算分子量 156 kDa
观测分子量140-150 kDa
GenBank蛋白编号NM_198229
基因名称 RGS12
Gene ID (NCBI) 6002
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO14924
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RGS12 antibody 85057-2-RRDownload protocol
IF protocol for RGS12 antibody 85057-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols