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RGS14 Monoclonal antibody, PBS Only

RGS14 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C1B2

Cat no : 67394-1-PBS

Print datasheet

Synonyms

Regulator of G-protein signaling 14, 1C1B2

验证数据展示

  • WB analysis of mouse brain using 67394-1-Ig (same clone as 67394-1-PBS)

    WB analysis of mouse brain using 67394-1-Ig (same clone as 67394-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67394-1-Ig (RGS14 antibody) at dilution of 1:13000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67394-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 67394-1-Ig (same clone as 67394-1-PBS)

    IHC staining of mouse brain using 67394-1-Ig (same clone as 67394-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67394-1-Ig (RGS14 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67394-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RGS14 antibody included
  • IHC staining of mouse brain using 67394-1-Ig (same clone as 67394-1-PBS)

    IHC staining of mouse brain using 67394-1-Ig (same clone as 67394-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67394-1-Ig (RGS14 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67394-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RGS14 antibody included
  • IF Staining of HeLa using 67394-1-Ig (same clone as 67394-1-PBS)

    IF Staining of HeLa using 67394-1-Ig (same clone as 67394-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RGS14 antibody (67394-1-Ig, Clone: 1C1B2 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67394-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 67394-1-Ig (same clone as 67394-1-PBS)

    FC experiment of HeLa using 67394-1-Ig (same clone as 67394-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug RGS14 Monoclonal antibody (67394-1-Ig, Clone:1C1B2) and CoraLite488-conjugated Donkey Anti-Mouse IgG(H+L)( SA00013-5)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67394-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy RGS14 Ready-To-Use IHC Kit. RGS14 primary antibody included.

产品信息

67394-1-PBS targets RGS14 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen RGS14 fusion protein Ag9477 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name regulator of G-protein signaling 14
Synonyms Regulator of G-protein signaling 14, 1C1B2
Calculated Molecular Weight 566 aa, 61 kDa
Observed Molecular Weight 61 kDa
GenBank Accession NumberBC014094
Gene Symbol RGS14
Gene ID (NCBI) 10636
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO43566
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RGS14, a member of the R12 subfamily of RGS proteins, is highly expressed in the brain and is a natural suppressor of CA2 hippocampal synaptic plasticity and learning and memory. RGS14 was first identified as a complex scaffolding protein with an unconventional domain structure that allows it to interact with various protein binding partners. RGS14 contains one RGS domain, two Raf-like Ras-binding domains (RBDs), and one GoLoco domain. The protein attenuates the signaling activity of G-proteins by binding, through its GoLoco domain, to specific types of activated, GTP-bound G alpha subunits. Acting as a GTPase activating protein (GAP), the protein increases the rate of conversion of the GTP to GDP.