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RGS14 Monoclonal antibody, PBS Only

RGS14 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C1B2

Cat no : 67394-1-PBS

Print datasheet

Synonyms

Regulator of G-protein signaling 14, 1C1B2

验证数据展示

  • WB analysis of mouse brain using 67394-1-Ig (same clone as 67394-1-PBS)

    WB analysis of mouse brain using 67394-1-Ig (same clone as 67394-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67394-1-Ig (RGS14 antibody) at dilution of 1:13000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67394-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 67394-1-Ig (same clone as 67394-1-PBS)

    IHC staining of mouse brain using 67394-1-Ig (same clone as 67394-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67394-1-Ig (RGS14 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67394-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RGS14 antibody included
  • IHC staining of mouse brain using 67394-1-Ig (same clone as 67394-1-PBS)

    IHC staining of mouse brain using 67394-1-Ig (same clone as 67394-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67394-1-Ig (RGS14 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67394-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RGS14 antibody included
  • IF Staining of HeLa using 67394-1-Ig (same clone as 67394-1-PBS)

    IF Staining of HeLa using 67394-1-Ig (same clone as 67394-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RGS14 antibody (67394-1-Ig, Clone: 1C1B2 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67394-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 67394-1-Ig (same clone as 67394-1-PBS)

    FC experiment of HeLa using 67394-1-Ig (same clone as 67394-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug RGS14 Monoclonal antibody (67394-1-Ig, Clone:1C1B2) and CoraLite488-conjugated Donkey Anti-Mouse IgG(H+L)( SA00013-5)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67394-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy RGS14 Ready-To-Use IHC Kit. RGS14 primary antibody included.

产品信息

67394-1-PBS targets RGS14 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen RGS14 fusion protein Ag9477 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name regulator of G-protein signaling 14
Synonyms Regulator of G-protein signaling 14, 1C1B2
Calculated Molecular Weight 566 aa, 61 kDa
Observed Molecular Weight 61 kDa
GenBank Accession NumberBC014094
Gene Symbol RGS14
Gene ID (NCBI) 10636
RRIDAB_2882638
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO43566
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RGS14, a member of the R12 subfamily of RGS proteins, is highly expressed in the brain and is a natural suppressor of CA2 hippocampal synaptic plasticity and learning and memory. RGS14 was first identified as a complex scaffolding protein with an unconventional domain structure that allows it to interact with various protein binding partners. RGS14 contains one RGS domain, two Raf-like Ras-binding domains (RBDs), and one GoLoco domain. The protein attenuates the signaling activity of G-proteins by binding, through its GoLoco domain, to specific types of activated, GTP-bound G alpha subunits. Acting as a GTPase activating protein (GAP), the protein increases the rate of conversion of the GTP to GDP.