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验证数据展示

  • WB analysis of mouse brain using 67394-1-Ig

    WB analysis of mouse brain using 67394-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67394-1-Ig (RGS14 antibody) at dilution of 1:13000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 67394-1-Ig

    IHC staining of mouse brain using 67394-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67394-1-Ig (RGS14 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RGS14 antibody included
  • IHC staining of mouse brain using 67394-1-Ig

    IHC staining of mouse brain using 67394-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67394-1-Ig (RGS14 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RGS14 antibody included
  • IF Staining of HeLa using 67394-1-Ig

    IF Staining of HeLa using 67394-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RGS14 antibody (67394-1-Ig, Clone: 1C1B2 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

  • FC experiment of HeLa using 67394-1-Ig

    FC experiment of HeLa using 67394-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug RGS14 Monoclonal antibody (67394-1-Ig, Clone:1C1B2) and CoraLite488-conjugated Donkey Anti-Mouse IgG(H+L)( SA00013-5)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

RGS14 Monoclonal antibody

RGS14 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 67394-1-Ig

产品说明书

CloneNo. 1C1B2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

Regulator of G-protein signaling 14, 1C1B2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, HeLa cells, HEK-293 cells, Jurkat cells, NIH/3T3 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RGS14 Ready-To-Use IHC Kit. RGS14 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:15000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67394-1-Ig targets RGS14 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RGS14 fusion protein Ag9477 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 regulator of G-protein signaling 14
别名 Regulator of G-protein signaling 14, 1C1B2
计算分子量 566 aa, 61 kDa
观测分子量 61 kDa
GenBank蛋白编号BC014094
基因名称 RGS14
Gene ID (NCBI) 10636
RRIDAB_2882638
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO43566
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RGS14, a member of the R12 subfamily of RGS proteins, is highly expressed in the brain and is a natural suppressor of CA2 hippocampal synaptic plasticity and learning and memory. RGS14 was first identified as a complex scaffolding protein with an unconventional domain structure that allows it to interact with various protein binding partners. RGS14 contains one RGS domain, two Raf-like Ras-binding domains (RBDs), and one GoLoco domain. The protein attenuates the signaling activity of G-proteins by binding, through its GoLoco domain, to specific types of activated, GTP-bound G alpha subunits. Acting as a GTPase activating protein (GAP), the protein increases the rate of conversion of the GTP to GDP.

实验方案

Product Specific Protocols
WB protocol for RGS14 antibody 67394-1-IgDownload protocol
IHC protocol for RGS14 antibody 67394-1-IgDownload protocol
IF protocol for RGS14 antibody 67394-1-IgDownload protocol
FC protocol for RGS14 antibody 67394-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols